Smoke saturated water promoted in vitro seed germination of an epiphytic orchid Oberonia ensiformis (Rees) Lindl.

Authors

  • Ravindra B. Malabadi Department of Agricultural Biotechnology, Bharati Vidyapeeth University, Pune-Satara Road, Katraj, Pune - 411046, Maharashtra, India
  • Neelambika T. Meti Department of Agricultural Biotechnology, Bharati Vidyapeeth University, Pune-Satara Road, Katraj, Pune - 411046, Maharashtra, India
  • Gangadhar S. Mulgund Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India
  • K. Nataraja Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India
  • S. Vijaya Kumar Department of Biotechnology, Madanapalle Institute of Technology and Science, Madanapalle- 517325, Chitoor, Andhra Pradesh, India

Keywords:

butenolide, 3-methyl-2H-furo [2, 3-c] pyran-2-one, PLB, protocorm-like body, TRIA, triacontanol, SSW, smoke saturated water

Abstract

This study highlights for the first time an in vitro seed germination of an epiphytic orchid Oberonia ensiformis (Rees) Lindl, from the Western Ghat Forest of Karnataka state,India promoted by smoke saturated water (SSW). High percentage germination (85%) andhigh percentage of plantlet recovery (73%) was achieved by culturing seeds on Mitra et al.(1976) basal medium supplemented with 10% (v/v) SSW. Well-rooted shoots weretransferred to pots containing charcoal chips, coconut husk and broken tiles (2:2:1) and 90%survived. Therefore, the role of SSW as a natural growth regulator at different stages ofdevelopment from seed germination to plant regeneration has been confirmed.

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Published

14-10-2012

How to Cite

B. Malabadi, R., T. Meti, N., S. Mulgund, G., Nataraja, K., & Kumar, S. V. (2012). Smoke saturated water promoted in vitro seed germination of an epiphytic orchid Oberonia ensiformis (Rees) Lindl. Research in Plant Biology, 2(5). Retrieved from https://updatepublishing.com/journal/index.php/ripb/article/view/2540

Issue

Vol. 2 No. 5 (2012)

Section

Articles

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