Synthesis of silver nanoparticles from in vitro derived plants and callus cultures of Costus speciosus (Koen.); Assessment of antibacterial activity

Abstract

This study demonstrates for the first time the efficiencies of in vitro derived plantsand callus cultures of Costus speciosus extract in the rapid biosynthesis of stable silve rnanoparticles. Synthesis of silver nanoparticles may be influenced directly or indirectly byphytochemicals in plants such as phenolics, flavonoids, and diosgenin compounds. Thisstudy also highlights a cost effective and environment friendly technique for green synthesisof silver nanoparticles. These silver nanoparticles were found to be highly toxic againstdifferent multi drug resistant clinical samples such as gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, and the gram-negative bacteria Escherichia coli and Klebsiella pneumoniae. This also provides evidence for developing large scale commercial productionof value-added products for biomedical / nanotechnology based industries, which is animportant step in the field of application of nanotechnology.