TDZ induced in vitro propagation of an epiphytic orchid Xenikophyton smeeanum (Reichb. f.)

Authors

  • Gangadhar S. Mulgund Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India
  • K. Nataraja Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India
  • Ravindra B. Malabadi Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India
  • S. Vijaya Kumar Department of Biotechnology, Madanapalle Institute of Technology and Science, Madanapalle-517325, Chitoor District, Andhra Pradesh, India

Keywords:

Conservation, micropropagation, Karnataka, native orchids, Western Ghat forests

Abstract

An efficient shoot regeneration of Xenikophyton smeeanum (Reichb. f.). was achievedusing shoot tip sections and thidiazuron (TDZ). Protocorm-like bodies (PLB’s) orproliferating shoot buds was observed when thin cell layers of shoot tip sections werecultured on Mitra et al. (1976) basal medium supplemented with 11.35μM thidiazuron.Shoots produced roots when cultured on Mitra et al (1976) basal medium supplementedwith 8.56 μM IAA. This simple protocol will be useful for large-scale propagation of nativeepiphytic orchid Xenikophyton smeeanum.

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Published

15-10-2011

How to Cite

S. Mulgund, G., Nataraja, K., B. Malabadi, R., & Vijaya Kumar, S. (2011). TDZ induced in vitro propagation of an epiphytic orchid Xenikophyton smeeanum (Reichb. f.). Research in Plant Biology, 1(4). Retrieved from https://updatepublishing.com/journal/index.php/ripb/article/view/2586

Issue

Vol. 1 No. 4 (2011)

Section

Articles

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