Evidence of WUSCHEL (WOX2) gene expression during induction of somatic embryogenesis from apical shoot buds of mature trees of Pinus roxburghii

Authors

  • Ravindra B. Malabadi Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India
  • K. Nataraja Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India
  • S. Vijaya Kumar Department of Biotechnology, Madanapalle Institute of Technology and Science, Madanapalle-517325, Chitoor District, Andhra Pradesh, India
  • Gangadhar S. Mulgund Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India

Keywords:

Apical buds, Chir pine, meristematic tissue, pines, somatic embryogenesis

Abstract

This study highlights for the first time the expression of transcription factor, WOX2(WUSCHEL homeobox 2) in the embryogenic tissue derived from the apical shoot buds of mature trees of 3 genotypes of P. roxburghii. Therefore, PrWOX2 could be used as a potential molecular signature for the identification of embryogenic cultures during the early development of somatic embryos in conifers. On the other hand, the non-embryogenic tissue of shoot bud cultures which were initiated without cold treatment (control) failed to showthe expression of WOX2. This clearly demonstrates the role of WOX2 in the somatic embryogenesis pathway and might be directly related to the stress conditions.

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Published

15-10-2011

How to Cite

B. Malabadi, R., Nataraja, K., Vijaya Kumar, S., & S. Mulgund, G. (2011). Evidence of WUSCHEL (WOX2) gene expression during induction of somatic embryogenesis from apical shoot buds of mature trees of Pinus roxburghii. Research in Plant Biology, 1(4). Retrieved from https://updatepublishing.com/journal/index.php/ripb/article/view/2592

Issue

Vol. 1 No. 4 (2011)

Section

Articles

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