Genetic relatidness and In vitro antimicrobial activities of alkaloids isolated from Indian varieties of Catharanthus roseus (L.)

Authors

  • Vardhan S Sindhu Mahavidyalaya, Centre for biotechnology, panchpaoli, Nagpur-440017, India.
  • A K Yadav Deen Dayal Upadhyay Gorakhpur University, Gorakhpur-273001. (U.P), India.
  • R K Suri Sindhu Mahavidyalaya, Centre for biotechnology, panchpaoli, Nagpur-440017, India.
  • B.B.S.P Nag Sindhu Mahavidyalaya, Centre for biotechnology, panchpaoli, Nagpur-440017, India.

Keywords:

Vinca rosea, Antimicrobial activity, RAPD.

Abstract

Catharanthus roseus (L.) is one of the most intensively studied for its anticancerous property but phytopathogenic activity of the plant has not been much studied. Callus induction from C. roseus was optimized by varying various harmone concentrations and the best result was obtanined in MS medium fortified with 2mgL-1 2, 4-D and 0.1 mgL-1 kinetin. Antimicrobial activity and minimum inhibitory concentration showed that the 20, 40, and 80% leaves, shoot, and root extract inhibits Gram positive B. subtilis and phytopathogenic fungus F. oxysporium, A. niger, Rhizoctonia solani, Colletotrichum falcatum, Cylindrocladium scoparium respectively. Genetic relatidness was determined by RAPD analysis revealed that, white and violet colored C.roseus flowered plant was closely related than rosy pink coloured flower.

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Author Biographies

Vardhan S, Sindhu Mahavidyalaya, Centre for biotechnology, panchpaoli, Nagpur-440017, India.

Sindhu Mahavidyalaya, Centre for biotechnology, panchpaoli, Nagpur-440017, India.

A K Yadav, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur-273001. (U.P), India.

Deen Dayal Upadhyay Gorakhpur University, Gorakhpur-273001. (U.P), India.

R K Suri, Sindhu Mahavidyalaya, Centre for biotechnology, panchpaoli, Nagpur-440017, India.

Sindhu Mahavidyalaya, Centre for biotechnology, panchpaoli, Nagpur-440017, India.

B.B.S.P Nag, Sindhu Mahavidyalaya, Centre for biotechnology, panchpaoli, Nagpur-440017, India.

Sindhu Mahavidyalaya, Centre for biotechnology, panchpaoli, Nagpur-440017, India.

Published

07-11-2012

How to Cite

S, V., Yadav, A. K., Suri, R. K., & Nag, B. (2012). Genetic relatidness and In vitro antimicrobial activities of alkaloids isolated from Indian varieties of Catharanthus roseus (L.). Current Botany, 3(4). Retrieved from https://updatepublishing.com/journal/index.php/cb/article/view/1420

Issue

Section

Regular Articles