Physiological and biochemical changes in desiccation sensitive curry leaf (Murraya koenigii (L.) Sprengel) seeds

  • K. Arulmoorthy, K. Raja Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India
  • S. Sundareswaran Seed Centre, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India


Curry leaf (Murraya koenigii (L.) Sprengel) is an important spice tree propagated by seeds. However, the seeds are sensitive to desiccation and considered as recalcitrant. Therefore, an experiment was conducted to analyze its level of desiccation and changes takes place during water loss. The results indicated that the reduction in seed moisture from 47.4 per cent leads to loss in viability. In which, the initial seed germination (100 %) declines slowly during desiccation and at the seed moisture content of 33.1 per cent it recorded 69 per cent germination at six days of exposure under ambient temperature (30±20C). Further moisture loss leads to drastic reduction in germination and hence, this could be the critical or lowest safe moisture content. In addition, the reduction in seedling vigour and enzymatic activity and increase in seed leachate were noticed during desiccation of the curry leaf seeds.

Keywords: Curry leaf, critical or lowest safe moisture content, recalcitrant seed, desiccation sensitive.


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1. Singh, S., More, P. K., & Mohan, S. M. Curry leaves (Murraya koenigii Linn. Sprengal) -a miracle plant. Indian J. Sci. Res, 2014; 4(1): 46-52.
2. Sivasubramaniam, K. and Selvarani, K. Studies on curry leaf (Murrya koenigii) seeds. Current Science, 2012; 103:883-885.
3. Raja, K., Palanisamy, V. and Selvaraju, P. Desiccation sensitivity of recalcitrant curry leaf (Murraya koenigii (Linn.) Sprengel) seeds. IPGRI/ Danida Forest Seed Centre (Denmark) Newsletter, 2001; 9:27-29.
4. ISTA, 2011. International rules for seed testing. International Seed Testing Association, Zurich, Switzerland.
5. Maguire, J.D. Speed of germination-Aid in selection and evaluation of seedling emergence and vigour. Crop Sci., 1962; 2: 176-177.
6. Abdul-Baki, A.A. and Anderson, J.D. Vigour determination in soya bean by multiple criteria. Crop Sci., 1973; 13: 630-633.
7. Presley, J.T. Relation to protoplast permeability of cotton seed viability and predisposition of seedling diseases. Plant Disease Reporter, 1958; 42: 582.
8. Somogyi, M. Note on sugar deterioration .J. Biol. Chem. 1952; 195: 19-23.
9. Yoshida, S, Forno, D. A. and Cock, J. H. Laboratory Manual for Physiological Studies of Rice. IRRI Publication, Philippines, 1971: Pp36-37.
10. Paul, A.K., Mukherji, S. and Sircar, S.M. Metabolic changes in rice seeds during storage. Indian J. Agri. Sci., 1970; 40: 1031–1036.
11. Malik, C.P. and Singh, M.B. In: Plant Enzymology and Histoenzymology. Kalyani Publishers, New Delhi, 1980:Pp. 53.
12. Panse, V.G. and Sukhatme, P.V. Statistical methods of agricultural workers. ICAR, Pub., New Delhi, 1967.
13. Roberts, E.H. Predicting the Storage Life of Seeds. Seed Sci. &Technol., 1973; 1: 499-514.
14. Chin H.F., Hor, Y.L and Mohd.Lassim, M.B. Identification of recalcitrant seeds. Seed Sci. &Technol., 1984; 12:429-436.
15. Gunasekaran, M. and Krishnasamy, V. Retention of vigour and viability of rubber (Hevea brasiliensis muell-Arg.) seeds. Neo Botanica, 1998; 6: 47-53.
16. ShyllaMerlin, J. and Palanisamy, V. Seed viability and storability of jack fruit (Artocarpus heterophyllus L.). Seed Res., 2000; 28: 166-170.
17. Raja, K., Palanisamy, V. Selvaraju, P. and Shanmugasundaram, K.A. Desiccation sensitivity of avocado (Persea americana Mill.) seeds. IPGRI/ Danida Forest Seed Centre (Denmark) Newsletter, 2001; 8: 22-24.
18. Raja, K., Palanisamy, V. and Selvaraju, P. Deteriorative changes associated with the loss of viability in desiccation – sensitive arecanut (Areca catechu L.) seed. Seed Sci. & Technol., 2005; 33: 177-184.
19. Vanitha, C., Ramamoorthy, K., Vijayakumar,A. and Sivasubramaniam, K. Moist sand conditioning to minimize the loss of viability in cocoa (Theobroma cacao Linn.) seed. Green page, 2005; 4:487-491.
20. Sivakumar, V., Warrier, R.R, Anandalakshmi, R., Tigabu, M., Odén, P.C., Vijayachandran, S.N., Geetha, S. and Singh, B.G. Germination requirements and storage behavior of Myristica dactyloides Gaertn seeds. Seed Sci. &Technol., 2006; 34:729-733.
21. Daboue, E.M.S, Neya, O., Nandziga, E.B., Ouedraogo, A., Ouedraogo, M. and Pasquini, S. Morphometric characteristics and desiccation response of Opilia amentacea seeds from Burkina Faso. Seed Sci. & Technol., 2017; 45: 354-367.
22. Tompsett, P.B. Desiccation and storage studies on Dipterocarpus robur L. seeds. Ann. Appl. Bio, 1987; 110:371-379
23. Yan, X. F., Cao, M. and H.L. Xu. Effects of desiccation and temperature on the germination of Shorea chinensis (Dipterocarpaceae) seeds. Seed Sci. & Technol., 2007; 5:232-236.
24. Xue, Peng and Wen, Bin. Desiccation tolerance of intermediate pomelo (Citrus maxima 'Mansailong') seeds following rapid and slow drying. Seed Sci. & Technol., 2018; 46: 511-519.
25. Li, A.H., Yang, J., He, H.J., Yang, X.Y. Seed desiccation tolerance and germination of a potentially threatened Chinese species Fosbergia shweliensis. Seed Sci. &Technol., 2013; 41: 479-482.
26. Keshavachandra, K. and Krishnakumar, G. Seed germination studies on Gymnacranthera canarica (King) Warb – A Vulnerable tree species of a highly threatened Myristica swamp ecosystem. J. Threatened Taxa, 2016; 8: 9009-9013.
27. Ellis, R.H., and Roberts, E.H. Towards a rational basis for testing seed quality. In: Seed Production (Ed.P.D. Hebblethwaite).Butter Worths, London, 1980, 605-635.
28. Pritchard, H.W., Haye, A. J., Wright, W. J. and Steadman,K.J., A comparative study of viability Inga species desiccation relation to the physical characteristics and chemical composition of the embryo. Seed Sci. &Technol., 1995; 23: 85-100.
29. Xia, Q.H, Chen, R.A and Fu, J.R. Effects of desiccation, temperature and other factors on the germination lychee (Litchi chinensis Sonn.) and longan (Euphoria longan Stend.) seeds. Seed Sci. &Technol., 1992; 20:119-127.
30. Heydecker, W. Vigour In: Viability of Seeds. (Ed. E.H. Roberts). Chapman and Hall, London, 1972: 209–252.
31. Loomis, W.D. and Battaile. Plant Phenol compounds and the isolation of plant enzymes. Phytochemistry, 1966; 5: 423-438.
32. Nautiyal, A.R. and Purohit,A.N., Seed viability in sal.III. Membrane distruption in ageing seeds of Shorea robusta. Seed Sci. & Technol., 1985; 13: 69-76.
33. Szezotka, Z. Amylolytic activity in acorns of Quercus borealis michx. during storage under controlled conditions . Arboretrum Hornickve,1974;19:129-134.
34. Clegg, J.S. Metabolism and the intracellular environment. The vicinal water network model. In: Cell – Associated Water, (Eds: W. Drost-Hansen and J.S. Clegg), Academic Press, New York, 1979: Pp363-413.
35. Berjak, P., Dini,M. and Pammenter, N.W. Possible mechanisms underlying the differing dehydration responses in recalcitrant and orthodox seeds: desiccation -associated sub cellular changes in propagates of Avicenia marina. Seed Sci. & Technol., 1984; 12: 365-384.
36. Pammenter, N.W. and P. Berjak. A review of recalcitrant seed physiology in relation to desiccation – tolerance mechanisms. Seed Sci., Res., 1999; 9: 13-37.
37. Boroughs, H. and Hunter, J.R. The effect of temperature on the germination of cocoa seeds. Proc. Amer. Soc. Hort. Sci., 1963; 82:222.
38. Bhattacharyya, A.K. and Basu, R.N. Retention of vigour and viability of jack fruit (Artocarpus heterophyllus Lam.) seed. Indian Agric., 1992; 36(2):65-74.
39. Bailey, S.D., Mitchell, D.G., Bazinet, M.L. and Weurman, C.J. 1962. Studies on the volatile components of different varieties of cocoa beans. J. Food Sci., 27:165-170.
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How to Cite
K. Raja, K. A., and S. Sundareswaran. “Physiological and Biochemical Changes in Desiccation Sensitive Curry Leaf (Murraya Koenigii (L.) Sprengel) Seeds”. Journal of Phytology, Vol. 11, no. 1,  , pp. 38-41, doi:
Research Article