Journal of Phytology

Survival of transport-stressed black pepper stem cuttings after glucose, sucrose, fructose, and IBA treatments

2024-08-04T17:19:50+0530

This study aimed to determine the survival, sprouting, and rooting performance of black pepper stem cuttings to glucose, sucrose, and fructose at different concentrations. The two-node stem cuttings were prepared from six to seven-node cuttings collected from mother vines of black pepper in the field from far and remote areas in Sarawak, Malaysia. The two-node cuttings were soaked completely for one hour with the following treatments: 1% glucose, 2% glucose, 3% glucose, 1% sucrose, 2% sucrose, 3% sucrose, 1% fructose, 2% fructose, 3% fructose, and 1000 ppm IBA served as a positive control, while filtered water was a negative control. All treated cuttings were sown in the polybags containing soil mix and monitored daily for up to 45 days. The study found that black pepper cuttings treated with a 3% glucose solution exhibited significantly better survival, sprouting, and rooting, total number of roots, and total length of roots at day 45, but they were about the same when soaked in IBA at 1000 ppm. The present study demonstrates that cuttings that have been in transit for a long time require sugar and hormone treatment once they arrive at a certain location to restore their vitality and survival.

Anti-advanced glycation end-products and antibacterial inhibitory activities of Neonauclea formicaria (Rubiaceae)

2024-06-18T11:58:41+0530

Neonauclea species have been studied for their phytochemical and potential pharmacological uses. However, many of its species still remained unexplored. The Philippine endemic Neonauclea formicaria has been documented for its ethnobotanical use, but little has been reported on its phytochemical and pharmacological profile. Hence, this study evaluates the total phenolic (TPC) and flavonoid (TFC) contents and assesses its ethanolic leaf extracts’ antiglycation, antioxidant, and antibacterial activities. N. formicaria leaf extracts at 1000 ppm gave 63.02±5.82 mg GAE/g DW TPC and 31.25±4.24 mg QE/g DW TFC. The extract showed a concentration-dependent activity in inhibiting AGE formation for the antiglycation assay, with an IC50 value of 2823.5 ppm. In antioxidant assay, the extract exhibited the highest TEAC value of 419.5±14.3 mg TE/kg DW at 250 ppm. Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa were shown to be the most susceptible to the leaf extract (1000 ppm), with zones of inhibition at 16.56±5.65 mm and 14.06±2.65 mm, respectively. The results highlight that N. formicaria bears promising properties with pharmacological and nutraceutical applications.