Light spectrum influences adventitious root formation and shoot growth in Euphorbia leucocephala Lotsy stem cuttings
DOI:
https://doi.org/10.25081/jfcls.2025.v11.9631Keywords:
Adventitious rooting, Steam cuttings, Plantlets, LED lightingAbstract
Euphorbia leucocephala Lotsy (commonly known as “pascuita”) is a popular ornamental species in Mexico, especially during the Christmas season. It is mainly propagated by stem cuttings; however, low rooting rates and poor plantlet quality limit its commercial potential. Light-emitting diode (LED) lighting has been shown to affect vegetative propagation in various species. This study evaluated the effects of different LED light spectra on adventitious root formation and shoot development in apical and basal cuttings of E. leucocephala. A split-plot design was used with four light treatments—white, red, and blue LEDs (90 μmol m-2 s-1, 16-hour photoperiod) and natural light—as main plots and cutting types (apical and basal) as subplots. After nine weeks, red LED light induced the highest rooting percentage (82%) and root development. Basal cuttings produced the longest roots (13.65 cm) and the highest number of roots per cutting (7.87). Shoot growth was also enhanced by LED lighting compared to natural light, particularly under red and blue light, with basal cuttings showing the most vigorous development. These results demonstrate that LED lighting, especially red and blue spectra, significantly improves adventitious rooting and the quality of plantlets obtained from stem cuttings of E. leucocephala.
Downloads
References
Abidi, F., Girault, T., Douillet, O., Guillemain, G., Sintes, G., Laffaire, M., Ahmed, H. Ben, Smiti, S., Huché-Thélier, L., & Leduc, N. (2013). Blue light effects on rose photosynthesis and photomorphogenesis. Plant Biology, 15(1), 67-74. https://doi.org/10.1111/j.1438-8677.2012.00603.x
Acevedo-Rodríguez, P., & Strong, M. T. (2012). Catalogue of seed plants of the West Indies. In Smithsonian Contributions to Botany (Vol. 98) Washington, DC: Smithsonian Institution. https://doi.org/10.5479/SI.0081024X.98.1
Alallaq, S., Ranjan, A., Brunoni, F., Novák, O., Lakehal, A., & Bellini, C. (2020). Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings. Frontiers in Plant Science, 11, 586140. https://doi.org/10.3389/fpls.2020.586140
Amri, E., Lyaruu, H. V. M., Nyomora, A. S., & Kanyeka, Z. L. (2010). Vegetative propagation of African Blackwood (Dalbergia melanoxylon Guill. & Perr.): Effects of age of donor plant, IBA treatment and cutting position on rooting ability of stem cuttings. New Forests, 39, 183-194. https://doi.org/10.1007/s11056-009-9163-6
Barbier, F. F., Lunn, J. E., & Beveridge, C. A. (2015). Ready, steady, go! A sugar hit starts the race to shoot branching. Current Opinion in Plant Biology, 25, 39-45. https://doi.org/10.1016/j.pbi.2015.04.004
Christiaens, A., Gobin, B., Van Huylenbroeck, J., & Van Labeke, M. C. (2019). Adventitious rooting of Chrysanthemum is stimulated by a low red:far-red ratio. Journal of Plant Physiology, 236, 117-123. https://doi.org/10.1016/j.jplph.2019.03.008
Daud, N., Faizal, A., & Geelen, D. (2013). Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light. In Vitro Cellular & Developmental Biology - Plant, 49(2), 183-190. https://doi.org/10.1007/s11627-012-9486-4
Davis, P. A., & Burns, C. (2016). Photobiology in protected horticulture. Food and Energy Security, 5(4), 223-238. https://doi.org/10.1002/fes3.97
Druege, U., Franken, P., & Hajirezaei, M. R. (2016). Plant hormone homeostasis, signaling, and function during adventitious root formation in cuttings. Frontiers in Plant Science, 7, 381. https://doi.org/10.3389/fpls.2016.00381
Druege, U., Hilo, A., Pérez-Pérez, J. M., Klopotek, Y., Acosta, M., Shahinnia, F., Zerche, S., Franken, P., & Hajirezaei, M. R. (2019). Molecular and physiological control of adventitious rooting in cuttings: phytohormone action meets resource allocation. Annals of Botany, 123(6), 929-949. https://doi.org/10.1093/aob/mcy234
García, G. D. V., & Alia-Tejacal, I. (2019). Manual de caracterización agronómica de pascuita (Euphorbia leucocephala Lotsy). Universidad Autónoma del Estado de Morelos.
Gil, C. S., Jung, H. Y., Lee, C., & Eom, S. H. (2020). Blue light and NAA treatment significantly improve rooting on single leaf-bud cutting of Chrysanthemum via upregulated rooting-related genes. Scientia Horticulturae, 274, 109650. https://doi.org/10.1016/j.scienta.2020.109650
Gil, C. S., Kwon, S. J., Jeong, H. Y., Lee, C., Lee, O. J., & Eom, S. H. (2021). Blue light upregulates auxin signaling and stimulates root formation in irregular rooting of rosemary cuttings. Agronomy, 11(9), 1725. https://doi.org/10.3390/agronomy11091725
Guan, L., Murphy, A. S., Peer, W. A., Gan, L., Li, Y., & Cheng, Z. M. (Max). (2015). Physiological and Molecular Regulation of Adventitious Root Formation. Critical Reviews in Plant Sciences, 34(5), 506-521. https://doi.org/10.1080/07352689.2015.1090831
Kulus, D., & Woźny, A. (2020). Influence of light conditions on the morphogenetic and biochemical response of selected ornamental plant species under in vitro conditions: A mini-review. Biotechnologia, 101(1), 75-83. https://doi.org/10.5114/bta.2020.92930
Livadariu, O., Maximilian, C., Rahmanifar, B., & Cornea, C. P. (2023). LED technology applied to plant development for promoting the accumulation of bioactive compounds: A review. Plants, 12(5), 1075. https://doi.org/10.3390/plants12051075
Manivannan, A., Soundararajan, P., Park, Y. G., Wei, H., Kim, S. H., & Jeong, B. R. (2017). Blue and red light-emitting diodes improve the growth and physiology of in vitro-grown carnations ‘Green Beauty’ and ‘Purple Beauty’. Horticulture Environment and Biotechnology, 58(1), 12-20. https://doi.org/10.1007/s13580-017-0051-2
Martínez-Villegas, Y. M., Andrade-Rodríguez, M., Colinas-León, M. T., Villegas-Torres, Ó. G., Castillo-Gutiérrez, A., & Alia-Tejacal, I. (2015). Culture media inorganic salts effect on pascuita (Euphorbia leucocephala Lotsy) growth. Revista Fitotecnia Mexicana, 38(4), 369-374.
Moon, H. K., Park, S.-Y., Kim, Y. W., & Kim, C. S. (2006). Growth of Tsuru-rindo (Tripterospermum japonicum) cultured in vitro under various sources of light-emitting diode (LED) irradiation. Journal of Plant Biology, 49(2), 174-179. https://doi.org/10.1007/BF03031014
Moosavi-Nezhad, M., Alibeigi, B., Estaji, A., Gruda, N. S., & Aliniaeifard, S. (2022). Growth, Biomass Partitioning, and Photosynthetic Performance of Chrysanthemum Cuttings in Response to Different Light Spectra. Plants, 11(23), 3337. https://doi.org/10.3390/plants11233337
Osterc, G., Petkovšek, M. M., & Stampar, F. (2016). Quantification of IAA metabolites in the early stages of adventitious rooting might be predictive for subsequent differences in rooting response. Journal of Plant Growth Regulation, 35(2), 534-542.
https://doi.org/10.1007/s00344-015-9559-9
Otiende, M. A., Fricke, K., Nyabundi, J. O., Ngamau, K., Hajirezaei, M. R., & Druege, U. (2021). Involvement of the auxin–cytokinin homeostasis in adventitious root formation of rose cuttings as affected by their nodal position in the stock plant. Planta, 254, 65. https://doi.org/10.1007/s00425-021-03709-x
Ouzounis, T., Rosenqvist, E., & Ottosen, C. O. (2015). Spectral effects of artificial light on plant physiology and secondary metabolism: A review. HortScience, 50(8), 1128-1135. https://doi.org/10.21273/HORTSCI.50.8.1128
Roth, O., Yechezkel, S., Serero, O., Eliyahu, A., Vints, I., Tzeela, P., Carignano, A., Janacek, D. P., Peters, V., Kessel, A., Dwivedi, V., Carmeli-Weissberg, M., Shaya, F., Faigenboim-Doron, A., Ung, K. L., Pedersen, B. P., Riov, J., Klavins, E., Dawid, C., … Weinstain, R. (2024). Slow release of a synthetic auxin induces formation of adventitious roots in recalcitrant woody plants. Nature Biotechnology, 42(11), 1705-1716. https://doi.org/10.1038/s41587-023-02065-3
Schroeter-Zakrzewska, A., & Pradita, F. A. (2021). Effect of colour of light on rooting cuttings and subsequent growth of chrysanthemum (Chrysanthemum × grandiflorum Ramat./Kitam.). Agriculture, 11(7), 671. https://doi.org/10.3390/agriculture11070671
Shen, Y., Fan, K., Wang, Y., Wang, H., Ding, S., Song, D., Shen, J., Li, H., Song, Y., Han, X., Qian, W., Ma, Q., & Ding, Z. (2022). Red and blue light affect the formation of adventitious roots of tea cuttings (Camellia sinensis) by regulating hormone synthesis and signal transduction pathways of mature leaves. Frontiers in Plant Science, 13, 943662. https://doi.org/10.3389/fpls.2022.943662
Wu, H. C., & Lin, C. C. (2012). Red light-emitting diode light irradiation improves root and leaf formation in difficult-to-propagate Protea cynaroides L. plantlets in vitro. HortScience, 47(10), 1490-1494. https://doi.org/10.21273/HORTSCI.47.10.1490
Published
How to Cite
Issue
Section
Copyright (c) 2025 Carlos de Jesús Morales-Becerril, María Teresa Colinas-León, Ramón Marcos Soto-Hernández, María Teresa Martínez-Damián, Natanael Magaña-Lira

This work is licensed under a Creative Commons Attribution 4.0 International License.