In vitro antioxidant, cytotoxicity and FTIR analysis of leaf extract of Ayapana triplinervis
DOI:
https://doi.org/10.25081/cb.2026.v17.9660Keywords:
Ayapana triplinervis, In vitro antioxidant, In vitro cytotoxicity, FTIR analysisAbstract
Ayapana triplinervis (Vahl) R.M. King & H. Robinson belongs to the family Asteraceae, is an erect, perennial, ethnomedicinal, ornamental herb and is widely used in various mystical religious rituals of the various traditional communities in Asia, Africa, and South America. The leaves are used as decoctions, teas, antiviral agents, blood coagulants, cardiac stimulants, expectorants, emetic laxatives, antiscorbutic and are widely used in wounds, bleeding stomachs, diarrhoea, etc. The main aim of the proposed work is the preliminary phytochemical screening, in vitro antioxidant, cytotoxicity and FTIR analysis of crude methanolic leaf extract of A. triplinervis. The preliminary phytochemical analysis reveals the presence of glycosides, alkaloids, terpenoids, phenols, flavonoids, tannins and saponins, whereas steroids were absent. In antioxidant analysis, the DPPH radical scavenging assay and the nitric oxide radical scavenging assay were selected. In both the assays, it was shown that the ability of the methanolic leaf extract had the highest scavenging effect on DPPH radical than that of nitric oxide radical, which was increasing with an increase in the concentration of the sample. In in vitro cytotoxic analysis, leaf extract showed potential cytotoxic activity against different cell lines like HCT 116 (Human Colon Cancer Cell Line) and MCF 7 (Human Breast Cancer Cell Line). The cytotoxic effect was expressed as IC50. The IC50 value against the HCT 116 cell line was 80.14±3.12 μg/mL whereas 94.02±2.43 μg/mL against the MCF7 cell line, which indicated that the crude leaf extract of A. triplinervis showed the highest cytotoxicity against selected cancer cell lines. The functional group identification of the active compounds in the plant samples based on the peak value is mainly done by FTIR analysis. This study revealed that crude leaf extract of A. triplinervis exhibits high antioxidant and cytotoxicity potentials and will be helpful for the sustainable utilization of the plant.
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