Phytochemical profiling and FTIR analysis of aqueous extracts from three selected ethnomedicinal plants of North East India
DOI:
https://doi.org/10.25081/cb.2025.v16.9117Keywords:
Bioactive, Drug, Functional groups, Medicinal, MetabolitesAbstract
Ethnomedicinal plants have played an important role in natural product research and drug discovery since antiquity. The current study used phytochemical screening and Fourier Transform Infrared Spectroscopy (FTIR) to analyze the leaves of three ethnomedicinal plants identified in Goalpara area of Assam. The investigation was carried out to characterize the crude extract and identify the functional groups in these plants. Phytochemical screening revealed the presence of many secondary plant metabolites in the examined plants, including alkaloids, glycosides, flavonoids, terpenoids, steroids, tannins and phenolic compounds. The FTIR spectroscopy study revealed the existence of several key functional groups. The presence of various functional groups such as C-I stretch, C=O stretch, C-H stretch, and C-N was confirmed by FT-IR analysis. As a result, the current study provides solid support and a foundation for using these plant species as herbal treatments for a variety of diseases. This study confirms that aqueous leaf extracts of Zanthoxylum oxyphyllum Edgew., Rotheca serrata (L.) Steane & Mabb., and Blumea lanceolaria (Roxb.) Druce contains numerous bioactive compounds, in addition to previously reported phytochemicals, which could be utilized in the development of plant-based drugs.
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References
Ahmadiani, A., Hosseiny, J., Semnanian, S., Javan, M., Saeedi, F., Kamalinejad, M., & Saremi, S. (2000). Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruit extract. Journal of Ethnopharmacology, 72(1-2), 287-292. https://doi.org/10.1016/S0378-8741(00)00222-1
Anis, M., Sharma, M. P., & Iqbal, M. (2000). Herbal ethnomedicine of the gwalior forest division in madhya pradesh, India. Pharmaceutical Biology, 38(4), 241-253. https://doi.org/10.1076/1388-0209(200009)3841-AFT241
Arunprasath, A., & Indhumathi, M. (2019). Identification of functional groups in Corbichonia decumbens by Fourier-Transform Infrared Spectroscopy. Journal of Drug Delivery & Therapeutics, 9(3), 583-587. https://doi.org/10.22270/jddt.v9i3-s.3003
Ashokkumar, R., & Ramaswamy, M. (2014). Phytochemical screening by FTIR spectroscopic analysis of leaf extracts of selected Indian medicinal plants. International Journal of Current Microbiology and Applied Sciences, 3(1), 395-406.
Atif, B. A., & Carol, S. F. (2000). Phytosterols as anticancer dietary components: evidence and mechanism of action. The Journal of Nutrition, 130(9), 2127-2130. https://doi.org/10.1093/jn/130.9.2127
Ayangla, N. W., Singh, N., & Kumar, A. (2016). Phytochemical analysis of plant species of genus Zanthoxylum. International Journal of Medicine and Pharmaceutical Science, 6(1), 1-8.
Baker, E. A. (1982). Chemistry and Morphology of Plant Epicuticular Waxes. In D. F. Cutler, K. L. Alvin & C. E. Price (Eds.), The Plant Cuticle (pp. 139-166) London, UK: Academic Press.
Batiha, G. E., Beshbishy, A. M., Alkazmi, L., Adeyemi, O. S., Nadwa, E., Rashwan, E., El-Mleeh, A., & Igarashi, I. (2020). Gas chromatography-mass spectrometry analysis, phytochemical screening and anti-protozoal effects of the methanolic Viola tricolor and acetonic Laurus nobilis extracts. BMC Complementary Medicine and Therapies, 20, 87. https://doi.org/10.1186/s12906-020-2848-2
Bouic, P. J. D. (2001). The role of phytosterols and phytosterols in immune modulation: a review of the past 10 years. Current Opinion Clinical Nutrition and Metabolic Care, 4(6), 471-475. https://doi.org/10.1097/00075197-200111000-00001
Boyce, P. W., & Christy, L. P. (2004). Applied pharmacology for veterinary technicians. (2nd ed). St. Luis, USA: WB Saunders Co.
Chandra, S. (2018). Fourier transform infrared (FT-IR) spectroscopic analysis of Nicotiana plumbaginifolia (Solanaceae). Journal of Medicinal Plants Studies, 7(1), 82-85.
Chen, M., Jin, H.-Z., Zhang, W.-D., Yan, S.-K., & Shen, Y.-H. (2009). Chemical constituents of plants from the genus Blumea. Chemistry and Biodiversity, 6(6), 809-817. https://doi.org/10.1002/cbdv.200800113
de Amorin, A., Borba, H. R., Carauta, J. P. P., Lopes, D., & Kaplan, M. A. C. (1999). Anthelmintic activity of the latex of Ficus species. Journal of Ethnopharmacology, 64(3), 255-258. https://doi.org/10.1016/S0378-8741(98)00139-1
Dhivya, S. M., & Kalaichelvi, K. (2017). UV-Vis Spectroscopic and FTIR analysis of Sarcostemma brevistigma, Wight. And Arn. International Journal of Current Pharmaceutical Research, 9(3), 46-49. https://doi.org/10.22159/ijcpr.2017.v9i3.18890
Eberhardt, T. L., Li, X., Shupe, T. F., & Hse, C. Y. (2007). Chinese tallow tree (Sapium Sebiferum) utilization: Characterization of extractives and cell-wall chemistry. Wood and Fiber Science, 39(2), 319-324.
Epand, R. F., Savage, P. B., & Epand, R. M. (2007). Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ceragenins). Biochimica et Biophysica Acta (BBA)-Biomembranes, 1768(10), 2500-2509. https://doi.org/10.1016/j.bbamem.2007.05.023
Evans, W. C. (2009). Trease and Evans Pharmacognosy. London, UK: Saunders Limited.
Fujimoto, Y., Soemartono, A., & Sumatra, M. (1988). Sesquiterpene lactones from Blumea balsamifera. Phytochemistry, 27(4), 1109-1111. https://doi.org/10.1016/0031-9422(88)80284-X
Furlan, C. P. B., Marques, A. C., Marineli, R. da. S., & Junior, M. R. M. (2013). Conjugated linoleic acid and phytosterols counteract obesity induced by high-fat diet. Food Research International, 51(1), 429-435. https://doi.org/10.1016/j.foodres.2012.12.023
Griffiths, P. R., & de Haseth, J. A. (1986). Fourier transform infrared spectroscopy. New York, UK: Wiley.
Gupta, A. K., Tandon, N., & Sharma, M. (2008). Review on Indian Medicinal Plants (Vol. 7, pp. 110-111) New Delhi, India: Indian Council of Medical Research.
Harborne, A. J. (1998). Phytochemical methods A guide to modern techniques of plant analysis. (3rd ed.). Dordrecht, Netherlands: Springer.
Hooker, J. D. (1875). The Flora of British India. London, UK: L. Reeve & Co, London.
Jain, J. B., Kumane, S. C., & Bhattacharya, S. (2016). Medicinal flora of Madhya Pradesh and Chattishgarh-A Review. Indian Journal of Traditional Knowledge, 5(2), 237-242.
Kamble, V., & Gaikwad, N. (2016). Fourier Transform Infrared Spectroscopy Spectroscopic Studies in Embelia ribes Burm. F.: A Vulnerable Medicinal Plant. Asian Journal of Pharmaceutical and Clinical Research, 9(3), 41-47. https://doi.org/10.22159/ajpcr.2016.v9s3.13804
Kavipriya, K., & Chandran, M. (2018). FTIR and GCMS Analysis of bioactive Phytocompounds in methonalic leaf extract of Cassia alata. Biomedical and Pharmalogy Journal, 11(1), 141-147. https://doi.org/10.13005/bpj/1355
Kebede, T., Gadisa, E., & Tufa, A. (2021). Antimicrobial activities evaluation and phytochemical screening of some selected medicinal plants: A possible alternative in the treatment of multidrug-resistant microbes. Plos One, 16(3), e0249253. https://doi.org/10.1371/journal.pone.0249253
Koshy, O., Thomas, S., & Subramanian, L. (2017). Green Synthesis of Silver Nanoparticles using Aqueous Plant Extracts and its Application as Optical Sensor. International Journal of Biosensors & Bioelectronics, 2(3), 82-85. https://doi.org/10.15406/ijbsbe.2017.02.00022
Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal, 2013, 162750. https://doi.org/10.1155/2013/162750
Lakshmi, N. D. M., Mahitha, B., Madhavi, T., & Sushmaa, N. J. (2015). Phytochemical screening and FTIR analysis of Clitoria ternatea leaves. International Journal of Scientific & Engineering Research, 6(2), 287-290.
Maitera, O. N., & Chukkol, I. B. (2016). Phytochemical and Fourier Transform Infrared Spectroscopy analysis of Faidherbia albida (Del) as a preservative agent. World Journal of Research and Review, 3(3), 25-29.
Maobe, M. A. G., & Nyarango, R. M. (2013). Fourier Transformer Infra-Red Spectrophotometer Analysis of Warburgia ugandensis Medicinal herb used for the Treatment of Diabetes, Malaria and Pneumonia in Kisii Region, Southwest Kenya. Global Journal of Pharmacology, 7(1), 61-68.
Marineli, R. da S., Furlan, C. P. B., Marques, A. C., Bicas, J., Pastore, G. M., & Marostica, M. R. (2012). Phytosterols: Biological effects and mechanisms of hypocholesterolemic action. In V. K. Gupta & M. G. Tuohy (Eds.), Biotechnology of Bioactive Compounds: Sources and Applications (pp. 565-581) New York, US: John Wiley & Sons, Ltd. https://doi.org/10.1002/9781118733103.ch23
Marinova, D., Ribarova, F., & Atanassova, M. (2005). Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal of University and Chemical Technology and Metallurgy, 40(3), 255-260.
Mugendhiran, S., & Sheeja, B. D. (2020). Evaluation of phytoconstituents of Erigeron canadensis L. by FTIR and GC-MS analysis. International Journal of Pharmaceutical Sciences and Research, 12(5), 2823-2834. https://doi.org/10.13040/IJPSR.0975-8232.12(5).2823-34
Munda, S., & Kakoti, B. B. (2017). Pharmacological evaluation of methanolic extract of Zanthoxylum oxyphyllum Edgew with special reference to anti-Inflammatory and analgesic activity. International Journal of Applied Biology and Pharmaceutical Technology, 8(4), 5-10.
Navarro, A., Heras, B. D. L., & Villar, A. (2001). Anti-inflammatory and immunomodulating properties of a sterol fraction from Sideritis foetens. Biological & Pharmaceutical Bulletin, 24(5), 470-473. https://doi.org/10.1248/bpb.24.470
Noviany, N., Amrulloh, M. H., Mohamad, R., Irawan, B., Kusuma, W. A., Hadi, S., Supriyanto, R., Nofiani, R., Hussin, M. H., & Yuwono, S. W. (2023). FTIR-Based metabolomics for characterization of antioxidant activity of different parts of Sesbania grandiflora plant. Sains Malaysiana, 52(1), 165-174.
Paul, K. R., Irudayaraj, V., Johnson, M., & Patric, D. R. (2011). Phytochemical and anti-bacterial activity of epidermal glands extract of Christella parasitica (L.) H. Lev. Asian Pacific Journal of Tropical Biomedicine, 1(1), 8-11. https://doi.org/10.1016/S2221-1691(11)60059-2
Peterson, J., Dwyer, J., Bhagwat, S., Haylowitz, D., Hoiden, J., Eldridge, A. L., Beecher, G., & Aladesanmi, J. (2005). Major flavonoids in dry tea. Journal of Food Composition and Analysis, 18(6), 487-501. https://doi.org/10.1016/j.jfca.2004.05.006
Radhakrishnan, S., Alarfaj, A. A., & Annadurai, G. (2015). Estimation of phytochemical analysis and invitro antioxidant activity of calotropis gigantea extract: wound healing activity and its biomedical application. International Journal of Pharmaceutical Sciences and Research, 6(7), 3053-3060.
Ramya, B., Seetharaman, S., Indra, V., Sheela, D., Perinbam, K., & Augustain, G. (2017). Phytochemical profiling of aqueous extracts of selected medicinal plants. Journal of Pharmaceutical and Scientific Innovation, 6(1), 14-16.
Riaz, M., Khalid, R., Afzal, M., Anjum, F., Fatima, H., Zia, S., Rasool, G., Egbuna, C., Mtewa, A. G., Uche, C. Z., & Aslam, M. A. (2023). Phytobioactive compounds as therapeutic agents for human diseases: A review. Food Science & Nutrition, 11(6), 2500-2529. https://doi.org/10.1002/fsn3.3308
Shah, T. K., Kumar, A., Tandel, R., Bhat, R. A. H., & Sarma, D. (2019). Identification of functional groups in Thymus linearis ethanol leaf extract through fourier transform infrared spectroscopy (FTIR). Journal of Pharmacognosy and Phytochemistry, 8(4), 3013-3015.
Singh, M. K., Khare, G., Iyer, S. K., Sharwan, G., & Tripathi, D. K. (2012). Clerodendrum serratum: A Clinical approach. Journal of Applied Pharmaceutical Science, 2(2), 11-15.
Sinha, M. K., & Sinha, D. (2013). Herbal medicinal plants of Koria district (C.G.) India. International Journal of Green and Herbal Chemistry, 2(4), 1114-1120.
Skoog, D. A., Holler, F. J., & Crouch, S. R. (2007). Principles of instrumental analysis. (6th ed.). Belmont, California: Brooks Cole.
Starlin, T., Prabha, P. S., Thayakumar, B. A., & Gopalakrishnan, V. K. (2019). Screening and GC-MS profiling of ethanolic extract of Tylophora pauciflora. Journal of Biomedical Informatics, 15(6), 425-429. https://doi.org/10.6026/97320630015425
Sulaiman, C. T., & Balachandran, I. (2012). Total phenolics and total flavonoids in selected Indian medicinal plants. Indian Journal of Pharmaceutical Sciences, 74(3), 258-260.
Surewicz, W. K., Mantsch, H. H., & Chapman, D. (1993). Determination of Protein Secondary Structure by Fourier Transform Infrared Spectroscopy: A Critical Assessment. Biochemistry, 32(2), 389-393. https://doi.org/10.1021/bi00053a001
Theja, I., & Kuber, B. R. (2023). Phytochemical screening and Fourier Transform IR Analysis of Ficus sagittifolia (Warburg Ex Mildbread and Burret) stem bark. International Journal of Pharmacy Research & Technology, 13(1), 73-78.
Vanamane, R. S., Vhankade, A. M., Bhosale, P. A., Salgar, P. S., & Manjunath, G. (2021). FTIR (Fourier transform infrared spectroscopy) spectroscopic analysis of dried leaf and fruit peel extract of Capparis divaricata lam. International Journal of Environment, Agriculture and Biotechnology, 6(6), 141-145. https://doi.org/10.22161/ijeab.66.17
Venkanna, B., Uma, A., Suvarnalaxmi, Ch., Chandrasekharnath, N., Prakasham, R. S., & Jayalaxami, L. (2013). Antimicrobial property of Datura leaf extract against methicillin- resistant Staphylococcus aureus isolated from urethral and skin suppurative infection. Current Trends in Biotechnology and Pharmacy, 7(3), 782-792.
Victoria, S. H., Das, S., Lalhlenmawia, H., Phucho, L., & Shantabi, L. (2012). Study of analgesic, antipyretic and anti-inflammatory activities of the methanolic extract of Blumea lanceolaria (Roxb.) Druce. International Journal of Life Sciences Biotechnology and Pharma Research, 1(3), 2250-3137.
Visveshwari, M., Subbaiyan, B., & Thangapandian, V. (2017). Phytochemical analysis, antibacterial activity, FTIR and GCMS analysis of Ceropegia juncea Roxb. International Journal of Pharmacognosy and Phytochemical Research, 9(7), 914-920. https://doi.org/10.25258/phyto.v9i07.11155
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