A comprehensive approach to characterizing bioactive compounds from Haplanthodes tentaculatus (L.) R. B. Majumdar using HR-LCMS and MTT assay
DOI:
https://doi.org/10.25081/cb.2024.v15.8910Keywords:
Anticancer, Bioactive chemicals, Haplanthodes tentaculatus, High-resolution liquid chromatography mass spectrometry (HR-LCMS), Medication developmentAbstract
The bioactive compounds in Haplanthodes tentaculatus (L.) R. B. Majumdar has demonstrated significant potential in addressing conditions like cancer, diabetes, inflammation, and microbial infections. This study employs high-resolution liquid chromatography mass spectrometry and network analysis to investigate these chemicals. The methanolic extract revealed 92 identified compounds, many of which exhibited inhibitory effects on MDA-MB-231 breast cancer cell growth. Aclacinomycin N, Ganoderic acid Mb, Cucurbitacin E, and Hydroquinidine displayed notable inhibitory effects. The extract demonstrated an IC50 value of 61.41±0.692 μg, showcasing its effectiveness in impeding cancer cell development. While promising, further research is needed to unravel the specific molecular pathways involved. The identification and characterization of distinct chemicals in the extract offer potential leads for medication development. This work underscores H. tentaculatus as a vital source of bioactive substances with potent anticancer properties, emphasizing the need for continued exploration in advancing cancer therapies.
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Banerjee, S., Kar, A., Mukherjee, P. K., Haldar, P. K., Sharma, N., & Katiyar, C. K. (2021). Immunoprotective potential of Ayurvedic herb Kalmegh (Andrographis paniculata) against respiratory viral infections – LC–MS/MS and network pharmacology analysis. Phytochemical Analysis, 32(4), 629-639. https://doi.org/10.1002/pca.3011
Bastipati, S. B., Kalyani, C., Tulasi, C. D. S. L. N., & Saida, L. (2021). Anticancer Activity of Elytraria acaulis L. Extracts on Triple Negative Breast Cancer Cell Line. International Journal of Pharmaceutical Investigation, 11(4), 354-357. https://doi.org/10.5530/ijpi.2021.4.63
Benali, T., Bakrim, S., Ghchime, R., Benkhaira, N., El Omari, N., Balahbib, A., Zengin, G., Hasan, M. M., Bibi, S., & Bouyahya, A. (2022). Pharmacological insights into the multifaceted biological properties of quinic acid. Biotechnology and Genetic Engineering Reviews, 40(4), 3408-3437. https://doi.org/10.1080/02648725.2022.2122303
Bhagya, N., & Chandrashekar, K. R. (2020). Identification and quantification of cytotoxic phenolic acids and flavonoids in Ixora brachiata by UHPLC-DAD and UHPLC-ESI-MS/MS. International Journal of Mass Spectrometry, 450, 116290. https://doi.org/10.1016/j.ijms.2020.116290
Bourhia, M., Bouothmany, K., Bakrim, H., Hadrach, S., Salamatullah, A. M., Alzahrani, A., Alyahya, H. K., Albadr, N. A., Gmouh, S., Laglaoui, A., El Mzibri, M., & Benbacer, L. (2021). Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds. Separations, 8(8), 114. https://doi.org/10.3390/SEPARATIONS8080114
Brown, J. S., Amend, S. R., Austin, R. H., Gatenby, R. A., Hammarlund, E. U., & Pienta, K. J. (2023). Updating the Definition of Cancer. Molecular Cancer Research, 21(11), 1142-1147. https://doi.org/10.1158/1541-7786.MCR-23-0411
Carocho, M., & Ferreira, I. C. F. R. (2013). The Role of Phenolic Compounds in the Fight against Cancer – A Review. Anti-Cancer Agents in Medicinal Chemistry, 13(8), 1236-1258. https://doi.org/10.2174/18715206113139990301
Conley, E. C. (1996). N-Methyl-D-aspartate (NMDA)-selective glutamate receptor–channels. Ion Channel Factsbook, 1, 140-233. https://doi.org/10.1016/B978-012184450-9/50008-9
Dziedziński, M., Kobus-Cisowska, J., & Stachowiak, B. (2021). Pinus Species as Prospective Reserves of Bioactive Compounds with Potential Use in Functional Food—Current State of Knowledge. Plants, 10(7), 1306. https://doi.org/10.3390/PLANTS10071306
Gómez-Aldapa, C. A., Rangel-Vargas, E., Torres-Vitela, M. R., Villarruel-López, A., Acevedo-Sandoval, O. A., Gordillo-Martínez, A. J., Godínez-Oviedo, A., & Castro-Rosas, J. (2018). Antibacterial Activities of Hibiscus sabdariffa Extracts and Chemical Sanitizers Directly on Green Leaves Contaminated with Foodborne Pathogens. Journal of Food Protection, 81(2), 209-217. https://doi.org/10.4315/0362-028X.JFP-17-053
India Biodiversity Portal. (n.d.). Species, Haplanthodes tentaculatus (L.) R.B. Majumdarx, Retrieved from https://indiabiodiversity.org/species/show/229874
Kerru, N., Singh, P., Koorbanally, N., Raj, R., & Kumar, V. (2017). Recent advances (2015-2016) in anticancer hybrids. European Journal of Medicinal Chemistry, 142, 179-212. https://doi.org/10.1016/J.EJMECH.2017.07.033
Kim, S., Kim, J., Kim, N., Lee, D., Lee, H., Lee, D.-Y., & Kim, K. H. (2020). Metabolomic Elucidation of the Effect of Sucrose on the Secondary Metabolite Profiles in Melissa officinalis by Ultraperformance Liquid Chromatography-Mass Spectrometry. ACS Omega, 5(51), 33186-33195. https://doi.org/10.1021/acsomega.0c04745
Miao, M., & Xiang, L. (2020). Pharmacological action and potential targets of chlorogenic acid. Advances in Pharmacology, 87, 71-88. https://doi.org/10.1016/bs.apha.2019.12.002
Mughees, M., Wajid, S., & Samim, M. (2020). Cytotoxic potential of Artemisia absinthium extract loaded polymeric nanoparticles against breast cancer cells: Insight into the protein targets. International Journal of Pharmaceutics, 586, 119583. https://doi.org/10.1016/j.ijpharm.2020.119583
Ngoc, T. M., Phuong, N. T. T., Khoi, N. M., Park, S. J., Kwak, H. J., Nhiem, N. X., Trang, B. T. T., Tai, B. H., Song, J.-H., Ko, H.-J., & Kim, S. H. (2019). A new naphthoquinone analogue and antiviral constituents from the root of Rhinacanthus nasutus. Natural Product Research, 33(3), 360-366. https://doi.org/10.1080/14786419.2018.1452004
Ord, T. (2008). The Scourge: Moral Implications of Natural Embryo Loss. The American Journal of Bioethics, 8(7), 12-19. https://doi.org/10.1080/15265160802248146
Qian, X., He, J., Zhao, Y., & Lin, M. (2016). Inhibition of p38 MAPK phosphorylation is critical for bestatin to enhance ATRA-induced cell differentiation in acute promyelocytic leukemia NB4 cells. American Journal of Therapeutics, 23(3), e680-e689. https://doi.org/10.1097/01.MJT.0000433950.01406.B3
Rindi, G., Klimstra, D. S., Abedi-Ardekani, B., Asa, S. L., Bosman, F. T., Brambilla, E., Busam, K. J., de Krijger, R. R., Dietel, M., El-Naggar, A. K., Fernandez-Cuesta, L., Klöppel, G., McCluggage, W. G., Moch, H., Ohgaki, H., Rakha, E. A., Reed, N. S., Rous, B. A., Sasano, H.,...Cree, I. A. (2018). A common classification framework for neuroendocrine neoplasms: an International Agency for Research on Cancer (IARC) and World Health Organization (WHO) expert consensus proposal. Modern Pathology, 31(12), 1770-1786. https://doi.org/10.1038/S41379-018-0110-Y
Seffrin, J. R., Hill, D., Burkart, W., Magrath, I., Badwe, R. A., Ngoma, T., Mohar, A., & Grey, N. (2009). It Is Time to Include Cancer and Other Noncommunicable Diseases in the Millennium Development Goals. CA: A Cancer Journal for Clinicians, 59(5), 282-284. https://doi.org/10.3322/caac.20033
Şenol, H., Tulay, P., Ergören, M. Ç., Hanoğlu, A., Çaliş, I., & Mocan, G. (2021). Cytotoxic Effects of Verbascoside on MCF-7 and MDA-MB-231. Turkish Journal of Pharmaceutical Sciences, 18(5), 637. https://doi.org/10.4274/TJPS.GALENOS.2021.36599
Singh, R., Dhiman, M., Saklani, A., Selvaraj, C. I., & Kate, A. S. (2022). Isolation and characterization of a novel flavanone glycoside from an endemic plant Haplanthodes neilgherryensis. Journal of Asian Natural Products Research, 24(1), 96-101. https://doi.org/10.1080/10286020.2021.1880394
Yang, Y., Zhou, H., Liu, W., Wu, J., Yue, X., Wang, J., Quan, L., Liu, H., Guo, L., Wang, Z., Lian, X., & Zhang, Q. (2018). Ganoderic acid A exerts antitumor activity against MDA-MB-231 human breast cancer cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway. Oncology Letters, 16(5), 6515-6521. https://doi.org/10.3892/OL.2018.9475
Yavuz, M., Şahin, B., Baykal, A. T., & Demircan, T. (2023). Hydroquinidine displays a significant anticarcinogenic activity in breast and ovarian cancer cells via inhibiting cell-cycle and stimulating apoptosis. Turkish Journal of Biology, 47(1), 44-60. https://doi.org/10.55730/1300-0152.2640
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