In vitro regeneration of Arisaema leschenaultii (Blume) using leaf explants
DOI:
https://doi.org/10.25081/cb.2024.v15.8617Keywords:
Arisaema leschenaultii, Leaf explant, MS medium, Growth regulators, AcclimatizationAbstract
A standard in vitro regeneration protocol from leaf explants was developed for a potential medicinal plant Arisaema leschenaultii. Maximum callus induction (78%) was achieved on MS medium fortified with BAP (1.5 mg/L) and NAA (0.9 mg/L). The maximum percentage of shoot proliferation (75%) was attained on MS medium supplemented with 2.0 mg/L of BAP and 0.5 mg/L of GA3. The highest root induction (70%) was achieved on MS medium prepared with the combination of NAA (1.5 mg/L) and IBA (0.6 mg/L). The well-rooted plantlets that successfully followed acclimatization in the green house survived. The present efficient and optimized protocol provides mass propagation as well as the possibility of germplasm conservation for this study species.
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Ahn, Y. J., Vang, L., McKeon, T. A., & Chen, G. Q. (2007). High - frequency plant regeneration through adventitious shoot formation in castor (Ricinus communis L.). In Vitro Cellular & Developmental Biology - Plant, 43, 9-15. https://doi.org/10.1007/s11627-006-9009-2
Alam, I., Sharmin, S. A., Mondal, S. C., Alam, M. J., Khalekuzzaman, M., Anisuzzaman, M., & Alam, M. F. (2010). In vitro micropropagation through cotyledonary node culture of castor bean (Ricinus communis L.). Australian Journal of Crop Science, 4(2), 81-84.
Boro, P., Sarma, S., Deka, A. C., & Kalita, M. C. (1998). Clonal propagation of Alternanthera sessilis - A biopharmaceutically potent herbal medicinal plant. Journal of Phytological Research, 11(2), 103-106.
Faisal, M., & Anis, M. (2003). Rapid mass propagation of Tylophora indica Merrill via leaf callus culture. Plant Cell, Tissue and Organ Culture, 75, 125-129. https://doi.org/10.1023/A:1025084203958
Gamborg, O. L., & Phillips, G. C. (1995). Laboratory facilities, operation and management. In O. L. Gamborg & G. C. Phillips (Eds.), Fundamental methods of plant cell, tissue and organ culture (pp. 3-20) Berlin, New York: Springer.
Husain, M. K., & Anis, M. (2006). Rapid in vitro propagation of Eclipta alba (L.) Hassk. through high frequency axillary shoot proliferation. Acta Physiologiae Plantarum, 28, 325-330. https://doi.org/10.1007/s11738-006-0028-8
Jebakumar, M., & Jayabalan, M. (2000). An efficient method for regeneration of plantlets from nodal explants of Prosalea corydifolia Linn. Plant Cell Biotechnology and Molecular Biology, 1, 37-40.
Magendiran, M., Vijayakumar, K. K., Thambiraj, J., & Mahendran, S. (2022a). Germplasm conservation of Phyllanthus virgatus G. Forst. by encapsulation of in vitro derived leaf segments. Current Botany, 13, 4-7. https://doi.org/10.25081/cb.2022.v13.7303
Magendiran, M., Vijayakumar, K. K., Thambiraj, J., & Mahendran, S., (2022b). Rapid in vitro regeneration of medicinal plant Achyranthes bidentata Blume from the leaf explant through callus culture. NOVY 1 MIR Research Journal, 7(6), 1-12.
Mathew, K. M. (1999). The flora of the Palani hills (Monocotyledons) (Vol. 3, pp. 1368-1371) Tiruchirapalli, Tamil Nadu: Rapinat Herbarium.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Raja, H. D., & Arockiasamy, D. I. (2008). In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants. Plant Tissue Culture and Biotechnology, 18(1), 1-6. https://doi.org/10.3329/ptcb.v18i1.3243
Ramanathan, T., Satyavani, K., & Gurudeeban, S. (2010). In vitro plant regeneration from leaf primordia of gum-bearing tree Aegle marmelos. Research Journal of Forestry, 4(4), 208-212.
Sujatha, M., & Reddy, T. P. (1998). Differential Cytokinin effects on the stimulation of in vitro shoot proliferation from meristematic explants of castor (Ricinus Communis L.). Plant Cell Reports, 17, 561-566. https://doi.org/10.1007/s002990050442
Thambiraj, J., & Paulsamy, S. (2012). Rapid in vitro multiplication of the ethnomedicinal shrub, Acacia caesia (L.) Willd. (Mimosaceae) from leaf explants. Asian Pacific Journal of Tropical Biomedicine, 2(S2), S618-S622. https://doi.org/10.1016/S2221-1691(12)60284-6
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Copyright (c) 2024 K. K. Vijayakumar, V. Vijayan, S. Nigesh
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