Foliar epidermal micromorphology of genus Glochidion J.R.Forst. & G.Forst. (Phyllanthaceae) by using light and electron microscopy
DOI:
https://doi.org/10.25081/cb.2024.v15.8609Keywords:
Glochidion, Leaf epidermis, Light Microscopy, Field Emission Scanning Electron Microscopy, Principal Component Analysis, TrichomeAbstract
The present study was conducted to compare both qualitative and quantitative characteristics of foliar epidermal micromorphology on some members of Glochidion in Assam. As taxonomic attributes, the foliar epidermal micromorphology study of nine taxa of both abaxial and adaxial surfaces was performed by using light microscopy (LM) and field emission scanning electron microscopy (FESEM). The result showed both amphistomatic and hypostomatic types of leaf surfaces. On the same surface of the leaf, multiple types of stomata were observed such as anomocytic, anisocytic, hemiparacytic, and paracytic types. Significant diversity and variations were observed in stomatal number, size, area, epidermal cell number, subsidiary cells, and trichomes. The stomatal index, stomatal shape, epidermal cell shape, length and width of the stomata, and trichomes showed variation among the studied taxa. Glands were absent in all studied members. Papillae and epicuticular wax crystals were observed in some taxa. In addition, a taxonomic key was also provided based on foliar leaf epidermal characteristics using qualitative and quantitative data from LM and FESEM. Based on quantitative data of foliar leaf micromorphology, principal component analysis (PCA) and cluster analysis were carried out to authenticate the micromorphological data. These would aid in the identification of taxa as well as in taxonomic delimitation.
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Adams, C. M., Caporn, S. J. M., & Hutchinson, T. C. (1990). Crystal occurrence and wax disruption on leaf surfaces of cabbage treated with simulated acid rain. The New Phytologist, 114(1), 147-158. https://doi.org/10.1111/j.1469-8137.1990.tb00385.x
Aldhebiani, A., & Jury, S. (2013). Anatomical studies on the genus Euphorbia L. Saudi Arabia (Subgenera: Triucalli, Ermophyton, Esula and Chamaesyce). International Research Journal of Plant Science, 4(6), 168-191.
Ayodele, A. E., & Olowokudejo, J. D. (2006). The family Polygonaceae in West Africa: Taxonomic significance of leaf epidermal characters. South African Journal of Botany, 72(3), 442-459. https://doi.org/10.1016/j.sajb.2005.12.009
Backer, C. A., & van den Brink, R. C. B. (1963). Glochidion. In Flora of Java (Vol. 1, pp. 460-464) Netherlands: Noordhoff.
Balakrishnan, N. P., & Chakrabarty, T. (2007). The Family Euphorbiaceae in India: A synopsis of its Profile, Taxonomy and Bibliography. Dehradun, India: Bishen Singh Mahendra Pal Singh.
Balakrishnan, N. P., Chakrabarty, T., Sanjappa, M., Lakshminarsimhan P., & Singh, P. (2012). Flora of India (Vol. 23) West Bengal, India: Botanical Survey of India.
Barthlott, W., Neinhuis, C., Cutler, D., Ditsch, F., Meusel, I., Theisen, I., & Wilhelmi, H. (1998). Classification and terminology of plant epicuticular waxes. Botanical Journal of the Linnean Society, 126(3), 237-260. https://doi.org/10.1111/j.1095-8339.1998.tb02529.x
Beille, L. (1927). Glochidion. In H. Lecomte (Eds.), Flor Generale de l’Indo-Chine (Vol. 5, pp. 608-628) Paris, France: Masson.
Bentham, G., & Hooker, J. D. (1862-1863). Genera Plantarum (Vol. 3) London: various publishers.
Blair, W. F., & Turner, B. L. (1972). The integrative approach to biological classification, In J. A. Behnke (Eds.), Challenging Biological Problems (pp. 193-217) New York, US: Oxford University Press.
Boulos, S. T., & Beakbane, A. B. (1971). A chemical method for separating leaf epidermis from mesophyll tissue. Journal of Botany of the United Arab Republic, 14, 317-322.
Chakrabarty, T., & Balakrishnan, N. P. (2018). Glochidion. In Indo-Burmese Phyllanthaceae: A taxonomic Revision (pp. 194-255) Dehra Dun, India: Bishen Singh Mahendra Pal Singh.
Chakrabarty, T., & Gangopadhyay, M. (1995). The genus Glochidion (Euphorbiaceae) in Indian Subcontinent. Journal of Economic and Taxonomic Botany, 19(1), 173-234.
Clark, S. H. (1986). Preservation of Herbarium Specimens: An Archive Conservator’s Approach. Taxon, 35(4), 675-682. https://doi.org/10.2307/1221610
Cotthem, W. R. J. V. (1970). A classification of stomatal types. Botanical Journal of the Linnean Society, 63(3), 235-246. https://doi.org/10.1111/j.1095-8339.1970.tb02321.x
da Silva, N. R., da Silva Oliveira, M. W., de Almeida Filho, H. A., Pinheiro, L. F. S., Kolb R. M., & Bruno, O. M. (2017). Automatic Leaf Epidermis Assessment Using Fourier Descriptors in Texture Images. Bio Protocol, 7(23), e2630. https://doi.org/10.21769/BioProtoc.2630
Davis, P. H., & Heywood, V. H. (1963). Principles of angiosperm taxonomy. London, UK: Oliver and Boyd.
Duarte-Silva, A. G., Carvalho-Silva, M., & Camara, P. E. A. S. (2013). Morphology and development of leaf papillae in the Pilotrichaceae. Acta Botanica Brasilica, 27(4), 737-742. https://doi.org/10.1590/S0102-33062013000400013
Eberly, D. (2008). The Area of Intersecting Ellipses. United States of America. Geometric Tools, Redmond WA 98052.
eFloras. (2008). Missouri Botanical Garden, St. Louis, MO & Harvard University Herbaria, Cambridge, MA. Retrieved from http://www.efloras.org
El-Taher, A. M., El Gendy, A. E. G., Alkahtani, J., Elshamy, A. I., & Abd-ElGawad, A. M. (2020). Taxonomic Implication of Integrated Chemical, Morphological, and Anatomical Attributes of Leaves of Eight Apocynaceae Taxa. Diversity, 12(9), 334. https://doi.org/10.3390/d12090334
Ensikat, H. J., Ditsche-Kuru, P., & Barthlott, W. (2011). Scanning electron microscopy of plant surfaces: simple but sophisticated methods for preparation and examination. In A. Mendez-Vliess & J. Diaz (Eds.), Microscopy: Science, Technology, Applications and Education (pp. 248-255) Badajoz, Spain: Formatex Research Center.
García-Gutiérrez, E., Ortega-Escalona, F., & Angeles, G. (2020). A novel, rapid technique for clearing leaf tissues. Applications in Plant Science, 8(9), e11391. https://doi.org/10.1002/aps3.11391
Granato, D., Santos, J. S., Escher, G. B., Ferreira, B. L., & Maggio, R. M. (2018). Use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) for multivariate association between bioactive compounds and functional properties in foods: A critical perspective. Trends in Food Science & Technology, 72, 83-90. https://doi.org/10.1016/j.tifs.2017.12.006
Hammer, O., Harper, D. A. T., & Ryan, P. D. (2001). PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica, 4, 1-9.
Hoffmann, P., Kathriarachchi, H., & Wurdack, K. J. (2006). A phylogenetic classification of Phyllanthaceae (Malpighiales; Euphorbiaceae sensu lato). Kew Bulletin, 61(1), 37-53.
Hooker, J. D. (1890). The Flora of British India (Vol. 5, pp. 305-327) London, UK: L. Reeve & Co.
IPNI. (2021). International Plant Name Index. The Royal Botanic Gardens, Kew, Harvard University Herbaria & Libraries and Australian National Herbarium. Retrieved from http://www.ipni.org
Jain, S. K., & Rao, R. R. (1977). A handbook of Field and Herbarium Technique. New Delhi, India: Today & Tomorrow Publication.
Kanjilal, U. N., Kanjilal, P. C., Dey, R. N., & Das, A. (1940). Flora of Assam (Vol. 4, pp. 179-189) Calcutta, India.
Khan, F., Yousaf, Z., Ahmed, H. S., Arif, A., Rehman, H. A., Younas, A., Rashid, M., Tariq, Z., & Raiz, N. (2014). Stomatal patterning: an important taxonomic tool for systematical studies of tree species of angiosperm. Annual Research & Review in Biology, 4(24), 4034-4053. https://doi.org/10.9734/ARRB/2014/10073
Koch, K., & Barthlott, W. (2006). Plant Epicuticular Waxes: Chemistry, Form, Self-Assembly and Function. Natural Product Communications, 1(11), 1067-1072. https://doi.org/10.1177/1934578X0600101123
Kong, M.-J., & Hong, S.-P. (2019). Leaf micromorphology of the Persicaria sect. Cephalophilon (Polygonaceae) and its systematic re-evaluation. Phytotaxa, 391(3), 167. https://doi.org/10.11646/phytotaxa.391.3.1
Lersten, N. R., & Curtis, J. D. (2001). Idioblasts and other unusual internal foliar secretory structures in Scrophulariaceae. Plant Systematics and Evolution, 227, 63-73. https://doi.org/10.1007/s006060170057
Li, P. T. (1994). Glochidion. Flora Republica Popularis Sinicae. Tomus 44. Angiospermae Dicotyledoneae Euphorbiaceae (pp. 133-162). Beijin, China: Science Press.
Li, P. T., & Gilbert, M. G. (2008). Glochidion. In Z. Y. Wu & P. H. Raven (Eds.), Flora of China (Vol. 2, pp. 193-202) Beijing, China: Science Press & Missouri Botanical Garden Press.
Metcalfe, C. R., & L. Chalk, L. (1950). Anatomy of Dicotyledons (Vol. 2) Oxford, England: Clarendon Press.
Moon, H.-K., Hong, S.-P., Smets, E., & Huysmans, S. (2009). Phylogenetic significance of leaf micromorphology and anatomy in the tribe Mentheae (Nepetoideae: Lamiaceae). Botanical Journal of the Linnean Society, 160(2), 211-231. https://doi.org/10.1111/j.1095-8339.2009.00979.x
Mukherjee, A., & Acharya, J. (2014). Contemplation of applications of scanning electron microscopy in taxonomy with special reference to Phermatology. The Biobrio, 1(1), 4-11.
Ndam, L. M., Mih, A. M., Tening, A. S., Fongod, A. G. N., Temenu, N. A., & Fujii, Y. (2015). Foliar micromorphology of Euphorbia golondrina l.c. wheeler (Euphorbiaceae) from Cameroon. International Journal of Current Research, 7(7), 18261-18267.
Nguyen, N. T. (2007). Glochidion. In Taxonomy of Euphorbiaceae in Vietnam (pp. 86-96). Hanoi, Vietnam: National University Publishers.
Pathan, A. K., Bond, J., & Gaskin, R. E. (2010). Sample preparation for SEM of plant surfaces. Materialstoday, 12(1), 32-43. https://doi.org/10.1016/S1369-7021(10)70143-7
Payne, W. W. (1978). A glossary of plant hair terminology. Brittonia, 30, 239-255. https://doi.org/10.2307/2806659
POWO. (2021). Plants of the Word Online. Facilitated by the Royal Botanical Gardens, Kew. Retrieved from http://www.plantsoftheworldonline.org/
Radford, A. E., Dickson, W. C., Massey, J. R., & Bell, C. R. (1974). Vascular Plants Systematics. New York, US: Harper and Row.
Razzaq, A., Shahid, S., Akram, M., Ashraf, M., Iqbal, S., Hussain, A., Zia, M., Qadri, S., Saher, N., Shahzad, F., Shah, A. N., Rehman, A., & Jacobsen, S.-E. (2021). Stomatal State Identification and Classification in Quinoa Microscopic Imprints through Deep Learning. Complexity, 2021, 9938013. https://doi.org/10.1155/2021/9938013
Robinson, C. B. (1909). Glochidion. Philippine Journal of Science, 4, 87-104.
Shah, S. N., Ahmad, M., Zafar, M., Malik, K., Rashid, N., Ullah, F., Zaman, W., & Ali, M. (2018). A light and scanning electron microscopic diagnosis of leaf epidermal morphology and its systematic implication in Dryopteridaceae: Investigating 12 Pakistani taxa. Micron, 111, 36-49. https://doi.org/10.1016/j.micron.2018.05.008
Shaw, H. K. A. (1981). The Euphorbiaceae of Sumatra. Kew Bulletin, 36(2), 239-374. https://doi.org/10.2307/4113612
Solihani, N. S., Noraini, T., Azahana, A., & Nordahlia, A. S. (2015). Leaf micromorphology of some Phyllanthus L. species (Phyllanthaceae). AIP Conference Proceedings, 1678(1), 020022.
Song, J.-H., & Hong, S.-P. (2017). The systematic implications of leaf micromorphological characteristics in the tribe Neillieae (Spiraeoideae, Rosaceae). Korean Journal of Plant Taxonomy, 47(3), 222-235. https://doi.org/10.11110/kjpt.2017.47.3.222
Stace, C. A. (1969). The Significance of the Leaf Epidermis in the Taxonomy of the Combretaceae III. The Genus Combretum in America. Brittonia, 21(2), 130-143. https://doi.org/10.2307/2805522
Thakur, H. A., & Patil, D. A. (2011). The foliar epidermal studies in some hitherto unstudied Euphorbiaceae. Current Botany, 2(4), 22-30.
Thakur, H. A., & Patil, D. A. (2014). Foliar Epidermal Studies of Plants in Euphorbiaceae. – Taiwania, 59, 59-70. https://doi.org/10.6165/tai.2014.59.59
The Angiosperm Phylogeny Group, Chase, M. W., Christenhusz, M. J. M., Fay, M. F., Byng, J. W., Judd, W. S., Soltis, D. E., Mabberley, D. J., Sennikov, A. N., Soltis, P. S., & Stevens, P. F. (2016). An update of the angiosperm phylogeny group classification for the orders and families of flowering plants: APG IV. Botanical Journal of the Linnean Society, 181(1), 1-20. https://doi.org/10.1111/boj.12385
Vislobokov, N. A., Fu, L.-F., Wei, Y.-G., & Nuraliev, M. S. (2021). Leaf epidermal micromorphology in Aspidistra (Asparagaceae): diversity and taxonomic significance. PhytoKeys, 185, 65-86. https://doi.org/10.3897/phytokeys.185.72259
Yao, G., Song, Z.-Q., Xue, B.-E., Shi, S., Li, Y.-L. & Luo, S.-X. (2020). Taxonomic revision of the genus Glochidion (Phyllanthaceae) in Taiwan, China. PhytoKeys, 159, 137-159. https://doi.org/10.3897/phytokeys.159.54839
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