Phytochemical analysis and antibacterial activity of Andrographis lineata Nees (Acanthaceae)
DOI:
https://doi.org/10.25081/cb.2024.v15.8577Keywords:
Andrographis lineata, Phytochemical analysis, Flavonoids, Phenols, Antibacterial activityAbstract
Andrographis lineata Nees is an erect herb and has been used as a traditional medicine against many diseases. The present study was to investigate the phytochemical analysis and antibacterial activity of A. lineata plant extracts. The methanolic extracts of A. lineata showed a variety of phytochemicals such as alkaloids, flavonoids, phenols, tannins, terpenoids etc. The quantitative estimation of major phytochemical constituents revealed maximum amount of phenolic content in the roots and alkaloids in both the stem and leaf. Correspondingly, the highest amount of tannins and terpenoids were recorded in the leaf and flavonoids in the root samples. Furthermore, the plant extracts of A. lineata showed antibacterial activity against both Gram-positive and Gram-negative bacterial strains. Comparatively, stem extract was effective against Streptococcus mutans (18.19±0.04 mm), leaf extract against Enterobacter faecalis (24.13±0.14 mm) and root extract against Bacillus subtilis (24.09±0.14 mm) at 30 μg/μL concentration. However, the leaf extract was more effective against Escherichia coli (25.13±0.16 mm), Pseudomonas aeruginosa (20.20±0.13 mm) and Klebsiella pneumoniae (18.18±0.17 mm) at 30 μg/μL concentration. The studies infer that the phytochemical constituents of A. lineata have antibacterial properties and these herbs may be considered as the medicinal plant for treating bacterial diseases.
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Abu-Rabia, A. (2005). Urinary diseases and Ethnobotany among pastoral nomads in the Middle East. Journal Ethnobiology and Ethnomedicine, 1, 4. https://doi.org/10.1186/1746-4269-1-4
Akbar, S. (2011). Andrographis paniculata: A review of pharmacological activities and clinical effects. Alternative Medicine Review: A Journal of Clinical Therapeutic, 16(1), 66-77.
Alagesaboopathi, C. (2011). Phytochemical analysis and antimicrobial evaluation of Andrographis lineata Nees leaves and stem extracts. African Journal of Pharmacy and Pharmacology, 5(9), 1190 - 1195. https://doi.org/10.5897/AJPP11.166
Ayyanar, M., Sankarasivaraman, K., & Ignacimuthu, S. (2008). Traditional herbal medicines used for the treatment of diabetes among two major tribal groups in South Tamil Nadu, India. Ethnobotanical Leaflets, 12, 276-280.
Azwanida, N. N. (2015). A review on the extraction methods uses in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants, 4(3), 196.
Bhattacharjee, I., Chatterjee, S. K., & Chandra, G. (2010). Isolation and identification of antibacterial components in seed extracts of Argemone mexicana L. (Papaveraceae). Asian Pacific Journal of Tropical Medicine, 3(7), 547-551. https://doi.org/10.1016/S1995-7645(10)60132-0
Bishnu, J., Sunil, L., & Anuja, S. (2009). Antimicrobial property of different medicinal plants: Ocimum sanctum, Cinnamomum zeylanicum, Xanthoxylumarmatumand Origanum majorana. Kathmandu University Journal of Science and Engineering Technology, 5(1), 143-150. https://doi.org/10.3126/kuset.v5i1.2854
Chaithada, P., Supapan, J., Rodthuk, P., & Chainarong, S. (2018). Total flavonoids, total phenolic content and antioxidant activity from fruits, leaves, twigs and flowers of Mesua ferrea L. Walailak Journal of Science and Technology, 15(4), 295-304. https://doi.org/10.48048/wjst.2018.3946
Chaudhary, P., Janmeda, P., Docea, A. O., Yeskaliyeva, B., Razis, A. F. A., Modu, B., Calina, D., & Sharifi-Rad, J. (2023). Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Frontiers in Chemistry, 11, 1158198. https://doi.org/10.3389/fchem.2023.1158198
De Zoysa, M. H. N., Rathnayake, H., Hewawasam, R. P., & Wijayaratne, W. M. D. G. B. (2019). Determination of In vitro antimicrobial activity of five Sri Lankan medicinal plants against selected human pathogenic bacteria. International Journal of Microbiology, 2019, 7431439. https://doi.org/10.1155/2019/7431439
Duffy, C. F., & Power, R. F. (2001). Antioxidant and antimicrobial properties of some Chinese plant extracts. International Journal of Antimicrobial Agents, 17(6), 527-529. https://doi.org/10.1016/S0924-8579(01)00326-0
Duraipandiyan, V., Ayyanar, M., &Ignacimuthu, S. (2006). Antimicrobial activity of some ethnomedicinal plants used by Paliyartribe from Tamil Nadu, India. BMC Complementary and Alternative Medicine, 6, 35. https://doi.org/10.1186/1472-6882-6-35
Fardiyah, Q., Ersam, T., Suyanta, Slamet, A., Suprapto & Kurniawan, F. (2020). New potential and characterization of Andrographis paniculata L. Ness plant extracts as photoprotective agent, Arabian Journal of Chemistry, 13(12), 8888-8897. https://doi.org/10.1016/j.arabjc.2020.10.015
Forman, H. J., & Zhang, H. (2021). Targeting oxidative stress in disease: promise and limitations of antioxidant therapy. Nature Reviews Drug Discovery, 20, 689-709. https://doi.org/10.1038/s41573-021-00233-1
Gamble, J. S. (1956). Flora of the Presidency of Madras. Botanical Survey of India, Government of India, Culcutta.
Gupta, C., Garg, A. P., & Gupta, S. (2010). Antimicrobial and phytochemical studies of fresh ripe pulp and dried unripe pulp of Mangifera indica (AMCHUR). Middle-East Journal of Science Research, 5(2), 75-80.
Harborne, J. B. (1984). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. (2nd ed). London, UK: Chapman and Hall.
Hassan, A., & Ullah, H. (2019). Antibacterial and antifungal activities of the medicinal plant Veronica biloba. Journal of Chemistry, 2019, 5264943. https://doi.org/10.1155/2019/5264943
Isah, T., Umar, S., Mujib, A., Sharma, M. P., Rajasekharan, P. E., Zafar, N., & Frukh, A. (2018). Secondary metabolism of pharmaceuticals in the plant in vitro cultures: strategies, approaches, and limitations to achieving higher yield. Plant Cell, Tissue and Organ Culture, 132, 239-265. https://doi.org/10.1007/s11240-017-1332-2
Kishore, P. H., Reddy, M. V. B., Reddy, M. K., Gunasekar, D., Caux, C., & Bodo, B. (2003). Flavonoids from Andrographis lineata. Phytochemistry, 63(4), 457-461. https://doi.org/10.1016/S0031-9422(02)00702-1
Kurek, J. (2019). Alkaloids - Their Importance in Nature and Human Life. London, UK: IntechOpen. https://doi.org/10.5772/intechopen.73336
León-Buitimea, A., Garza-Cárdenas, C. R., Garza-Cervantes, J. A., Lerma-Escalera, J. A., & Morones-Ramírez J. R. (2020). The demand for new antibiotics: Antimicrobial peptides, nanoparticles, and combinatorial therapies as future strategies in antibacterial agent design. Frontiers in Microbiology, 11, 1669. https://doi.org/10.3389/fmicb.2020.01669
Li, M., Luo, Q., Tao, Y., Sun, X., & Liu, C. (2022). Pharmacotherapies for drug induced liver injury: A current literature review. Frontiers in Pharmacology, 12, 806249. https://doi.org/10.3389/fphar.2021.806249
Manandhar, S., Luitel, S., & Dahal, R. K. (2019). In vitro antimicrobial activity of some medicinal plants against human pathogenic bacteria. Journal of Tropical Medicine, 2019, 1895340. https://doi.org/10.1155/2019/1895340
Mukherjee, P. K., Kumar, N., Mal, M., & Houghton, P. J. (2007). Acetylcholinesterase inhibitors from plants. Phytomedicine, 14(4), 289-300. https://doi.org/10.1016/j.phymed.2007.02.002
Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: an overview. Journal of Nutritional Science, 5, e47. https://doi.org/10.1017/jns.2016.41
Pandey, J., Saini, V. K, & Raja, W. (2019). Evaluation of phytochemical analysis of Andrographis paniculata leaf and stem extract. World Journal of Pharmaceutical & Life Sciences, 5(2), 188-190.
Parsaeimehr, A., Martinez-Chapa, S. O., & Parra-Saldivar, R. (2017). Medicinal Plants Versus Skin Disorders: A Survey From Ancient to Modern Herbalism. In K. Kon & M. Rai (Eds.), Clinical Microbiology: Diagnosis, Treatments and Prophylaxis of Infections - The Microbiology of Skin, Soft Tissue, Bone and Joint Infections (Vol. 2, pp. 205-221) Cambridge, United States: Academic Press. https://doi.org/10.1016/B978-0-12-811079-9.00013-6
Polash, S. A., Saha, T., Hossain, M. S., & Sarker, S. R. (2017). Investigation of the phytochemicals, antioxidant, and antimicrobial activity of the Andrographis paniculata leaf and stem extracts. Adv. Bioscience and Biotechnology, 8(5), 149-162. https://doi.org/10.4236/abb.2017.85012
Polshettiwar, S. A., & Ganjiwale, R. O. (2007). Spectrophotometric estimation of total tannins in some ayurvediceye drops. Indian Journal of Pharmaceutical Sciences, 69(4), 574-576.
Rajendran, P., Nandakumar, N., Rengarajan, T., Palaniswami, R., Gnanadhas, E. N., Lakshminarasaiah, U., Gopas, J., & Nishigaki, I. (2014). Antioxidants and human diseases. Clinica Chimica Acta, 436, 332-347. https://doi.org/10.1016/j.cca.2014.06.004
Rang, H. P., Dale, M. M., & Ritte, J. M. (2003). Pharmacology. (4th ed.). Melbourne, Australia: Churchill Livingstone.
Rashrash, M., Schommer, J. C., & Brown, L. M. (2017). Prevalence and predictors of herbal medicine use among adults in the United States. Journal of Patient Experience, 4(3), 108-113. https://doi.org/10.1177/2374373517706612
Renugadevi, G., Ramanathan, T., Shanmuga, P. R., Thirunavukkarasu, P. (2013). Studies on effects of Andrographis paniculata (Burm.F.) and Andrographis lineata Nees (Family: Acanthaceae) extracts against two mosquitoes Culex quinquefasciatus (Say.) and Aedes aegypti (Linn.). Asian Pacific Journal of Tropical Medicine, 6, 176-179.
Safari, M., Ahmady-Asbchin, S., & Zamanifar, P. (2019). In vitro evaluation of antimicrobial activities from aqueous and methanolic extracts of cyanobacteria. European Journal of Biological Research, 9(3), 184-192. https://doi.org/10.5281/zenodo.3463632
Sangameswaran, B., Reddy, T. C., & Jayakar, B. (2008). Hepatoprotective effect leaf extract of Andrographis lineata Nees on liver damage caused by Carbon Tetrachloride in rats. Phytotherapy Research, 22(1), 124-126. https://doi.org/10.1002/ptr.2250
Sareer, O., Ahmad S., & Umar, S. (2014). Andrographis paniculata: a critical appraisal of extraction, isolation and quantification of andrographolide and other active constituents. Natural Product Research, 28(23), 2081-2101. https://doi.org/10.1080/14786419.2014.924004
Saxena, M., Saxena, J., Nema, R., Singh, D., & Gupta, A. (2013). Phytochemistry of medicinal plants. Journal of Pharmacognosy and Phytochemistry, 1(6), 168-182.
Sheeja, K., & Kuttan, G. (2007). Activation of cytotoxic and lymphocyte responses and attenuation of tumor growth in vivo by Andrographis paniculata extract and andrographolide. Immunopharmacology and Immunotoxicology, 29(1), 81-93. https://doi.org/10.1080/08923970701282726
Singh, D. K., Srivastava, B., & Sahu, A. (2004). Spectrophotometric determination of Rauwolfia alkaloids: estimation of reserpine in pharmaceuticals. Analytical Sciences, 20, 571-573. https://doi.org/10.2116/analsci.20.571
Singleton, V. L., Orthofer R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-179. https://doi.org/10.1016/S0076-6879(99)99017-1
Tungmunnithum, D., Thongboonyou, A., Pholboon, A., & Yangsabai, A. (2018). Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines, 5(3), 93. https://doi.org/10.3390/medicines 5030093
Umadevi, M., Bindhu, M. R., & Sathe, V. (2013). A novel synthesis of mallic acid capped silver nanoparticles using Solanum lycopersicum fruit extract. Journal of Material Science and Technology, 29(4), 317-322. https://doi.org/10.1016/j.jmst.2013.02.002
Welz, A. N., Emberger-Klein, A., & Menrad, K. (2018). Why people use herbal medicine: insights from a focus-group study in Germany. BMC Complementary Alternative Medicine, 18, 92. https://doi.org/10.1186/s12906-018-2160-6
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