Comparative cytotoxicity of in vitro and field grown shoots of Withania somnifera in Caenorhabditis elegans model

Authors

  • Krishnapriya Santhanu Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science & Higher Education for Women, Coimbatore-641043, Tamil Nadu, India
  • D. Parameshwari Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science & Higher Education for Women, Coimbatore-641043, Tamil Nadu, India
  • Kanimozhi Natarajan Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science & Higher Education for Women, Coimbatore-641043, Tamil Nadu, India
  • Sangeetha Vinod Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science & Higher Education for Women, Coimbatore-641043, Tamil Nadu, India
  • Kalaiselvi Senthil Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science & Higher Education for Women, Coimbatore-641043, Tamil Nadu, India

DOI:

https://doi.org/10.25081/cb.2022.v13.8016

Keywords:

W. Somnifera, toxicity, C. elegans, in vitro cultures

Abstract

Indian ginseng, also known as Withania somnifera, is a popular medicinal plant used as a domestic treatment for a number of age-related illnesses. The field grown WS roots are referred as as a Rasayana (Rejuvenator) medication in the traditional Ayurvedic medicine of India. It has been utilized as the main component in many formulations to help slow down the aging process, manage stress, and be a remarkable neuroprotectant. The quantity and quality of traditionally grown plants, however, provide a considerable hurdle to their use in herbal-based products. The objective of this study was to determine the toxicity of shoots of in vitro developed W. somnifera, in Caenorhabditis elegans model and to compare the toxicological effect with that of plant shoots grown in the field. We found that biosafety is strictly concentration dependent. It was clear from the results that 250 µg/µL of W. somnifera shoot extract exhibited maximum viability for wild type animals. 

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Published

20-10-2022

How to Cite

Santhanu, K., Parameshwari, D., Natarajan, K., Vinod, S., & Senthil, K. (2022). Comparative cytotoxicity of in vitro and field grown shoots of Withania somnifera in Caenorhabditis elegans model. Current Botany, 13, 70–75. https://doi.org/10.25081/cb.2022.v13.8016

Issue

Volume 13, 2022

Section

Regular Articles

Copyright (c) 2022 Krishnapriya Santhanu, D. Parameshwari, Kanimozhi Natarajan, Sangeetha Vinod, Kalaiselvi Senthil

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This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License.