Germplasm conservation of Phyllanthus virgatus G. Forst. by encapsulation of In vitro derived leaf segments
DOI:
https://doi.org/10.25081/cb.2022.v13.7303Keywords:
Phyllanthus virgatus, Micropropagation, Callus culture, OrganogenesisAbstract
The present study describes an efficient plant regeneration system for in vitro propagation of Phyllanthus virgatus through leaf explant. The leaf explants were cultured on Murashige & Skoog (MS) medium supplemented with BAP (1.5 mg L-1) and NAA (0.9 mg L-1 for efficient callus induction (82.26%). The maximum percentage of shoots (77.85%) was developed from BAP (2.0 mg L-1) and GA3 (0.5 mg L-1). The efficient rooting response (76.28%) was noted on a nutrient medium containing NAA (1.5 mg L-1) and IBA (0.2 mg L-1). Regenerated plantlets were successfully acclimatized and hardened off inside the culture room and then transferred to green house with a 72% survival rate.
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Arya, H. C., & Shekawat, N, S. (1987). In: Proceedings of the symposium on plant cell and tissue culture of economically important plants. (Ed.), Reddy GM. Hyderabad, India
Chitra, R., Rajamani, K., & Vadivel, E., (2009). Regeneration of plantlets from leaf and internode explants of Phyllanthus amarus Schum and Thonn. African Journal Biotechnology, 8(10), 2209-2211.
Coleman, G. D., & Ernst, S. G., (1990). Axillary shoot proliferation and growth of Populus deltoids shoot culture. Plant Cell Report, 9, 165-167. https://doi.org/10.1007/BF00232096
Dewan, A., Nanda, K., & Gupta, S.C. (1992). In vitro micro-propagation of Acacianilotica subsp. indica Brenan via cotyledonary nodes. Plant Cell Report, 12, 18-21. https://doi.org/10.1007/BF00232415
Faheem, M., Singh, S., Tanwer, B. S., Khan, M., & Shahzad, A. (2011). In vitro regeneration of multiplication shoots in Catharanthus roseus - an important medicinal plant. Advances in Applied Science Research, 2(1), 208-213.
Galiana, A., Tibok, A., & Duhoux, E. (1991). In vitro propagation of the nitrogen-fixing tree-legume Acacia mangium Willd.. Plant and Soil, 135, 151–159 (1991). https://doi.org/10.1007/BF00010902.
Gaspar, T. H., Kevers, C., Faivre-Rampant, O., Crevecoeur, M., Penel, C. L., Greppin H., & Dommes, J. (2003). Changing concepts in plant hormones action. In vitro Cellular Developmental Biology-Plant, 39, 85 - 106. https://doi.org/10.1079/IVP2002393
Gaspar, T. H., Kevers, C., Penel, C., Greppin, H., Reid, D. M., & Thorpe, T. A. (1996). Plant hormones and plant growth regulators in plant tissue culture. In vitro Cellular Developmental Biology – Plant, 32, 272 - 289. https://doi.org/10.1007/BF02822700
Jones, T. C., Batchelor, C. A., & Harris, P. J. C. (1990). In vitro culture and propagation of Acacia species (A. bivenosa, A. holosericea, A. salicina, A. saligna and A. sclerosperma), International Tree Crops Journal, 6(2-3), 183-192. https://doi.org/10.1080/01435698.1990.9752883
Murashige, T., & Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Senthilkumar, P., Paulsamy, S., & Mallikadevi, T. (2009). In vitro regeneration of an important medicinal plant, Ophiorrhiza mungos L. Journal of Agriculture, Biotechnology and Ecology, 2(3), 322-330.
Sudipta, K. M., Kumara Swamy, M., Balasubramanaya, S., & Anuradha, M. (2011). Cost effective approach for in vitro propagation of Leptadenia reticulate Wight & Arn. - A threatened plant of medicinal importance. Journal of Phytology, 3(2), 72 - 77.
Uddin, M. S., Chowdhury, M. S. H., Khan, M. M. M. H., Uddin, M. B., Ahmed, R., & Baten, M. D. A. (2006). In vitro propagation of Stevia rebaudianabert in Bangladesh. African Journal of Biotechnology, 5(13), 1238-1240.
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Copyright (c) 2022 M. Magendiran, K. K. Vijayakumar, J. Thambiraj, S. Mahendran
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