Callus induction and axillary shoot formation in Asparagus racemosus Willd.

Authors

  • Neelofer Nabi Department of Botany, University of Kashmir, Srinagar, J&K-190006, India & Department of Botany, Jamia Hamdard, New Delhi-110062, India
  • Seema Singh Department of Botany, University of Kashmir, Srinagar, J&K-190006, India
  • Peer Saffeullah Department of Botany, Jamia Hamdard, New Delhi-110062, India

DOI:

https://doi.org/10.25081/cb.2020.v11.6036

Keywords:

2,4-D, Asparagus, BAP, Callus induction, NAA, Shoot formation

Abstract

An experiment was performed to establish a regeneration protocol for an important medicinal plant, Asparagus racemosus. In the present investigation, nodal and internodal explants were employed for callus induction and axillary shoot formation. Maximum callus induction frequency was found on MS medium fortified with 2,4-D (1.0 mg/L) along with NAA (1.0 mg/L) and BAP (0.5 mg/L). However, individual effects of 2,4-D or NAA with BAP showed least callus induction. The higher concentrations of 2,4-D and BAP decreased the response of explants. However, maximum axillary shoot formation was observed on MS medium adjuvanted with BAP (2.0 mg/L) and NAA (0.5 mg/L).

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Published

05-09-2020

How to Cite

Nabi, N., Singh, S., & Saffeullah, P. (2020). Callus induction and axillary shoot formation in Asparagus racemosus Willd. Current Botany, 11, 148–151. https://doi.org/10.25081/cb.2020.v11.6036

Issue

Volume 11, 2020

Section

Regular Articles