Determination of DNA content and relative 2C genome sizes of some promising commercial varieties of sugarcane using flow cytometer

Authors

  • A. Mondal S.K. Ghosal POSTGRADUATE DEPARTMENT OF BOTANY,HOOGHLY MOHSIN COLLEGE, BURDWAN UNIVERSITY,CHINSURAH ,HOOGHLY,WEST BENGAL, INDIA
  • T. Pal Kalyan Kumar De

DOI:

https://doi.org/10.19071/cb.2017.v8.3207

Abstract

In the present study, 2C DNA content and the genome sizes (in picograms-pg and megabase pairs-Mbp respectively) of 19 promising commercial varieties of sugarcane, the derivatives of man-made interspecific hybrids between cultivated and wild species were analyzed using flow cytometry. In this work, 2C nuclear DNA content was determined. Knowing the 2C nuclear DNA content, the unknown chromosome numbers of the varieties could be predicted. Large differences (65 % variation) in DNA content (2C) of 19 varieties were detected, ranging, from 3.80 pg to 10.96 pg, which corresponds to a genome size ranging from 3724.00 Mbp to 10740.80 Mbp due to the variation of ploidy level and are considered the most complex genomes among crop plants. However, the relationship between chromosome number and genome size was highly significant (P < 0.001). In the present study, internode diameter, Sugar juice content and cane yield/ha are also positively correlated with DNA content. The estimated genome sizes would also yield information critical for sugarcane breeding and genome sequencing programs.                                

Keywords: Genome size, Sugarcane varieties, Flow cytometry, DNA content.

Downloads

Download data is not yet available.

Author Biography

A. Mondal S.K. Ghosal, POSTGRADUATE DEPARTMENT OF BOTANY,HOOGHLY MOHSIN COLLEGE, BURDWAN UNIVERSITY,CHINSURAH ,HOOGHLY,WEST BENGAL, INDIA

POSTGRADUATE DEPARTMENT OF BOTANY

PROFESSOR

References

Arumuganathan K, Earle ED. Nuclear DNA content of some important plant species. Plant Mol Bio Rep 1991a; 9: 211-5.

Arumuganathan K, Earle ED. Estimation of nuclear DNA contents of plants by flow cytometry. Plant Mol Bio Rep 1991b; 9: 229-41.

Bennet MD, Leitch IJ. Nuclear DNA amounts in angiosperms. Ann Bot 1995; 76: 113-76.

Bennet MD, Smith IJ. Nuclear DNA amounts in angiosperms. Philos Trans R Soc Lond 1976; B274: 227-74.

Bennett MD. Nuclear DNA content and minimum generation time in herbaceous plants. Proc Roy Soc Lond 1972; B181: 109-35.

Biradar DP, Bullock DG, Rayburn AL. Nuclear DNA amount growth and yield parameters in maize. Theor and Appl Genet 1994; 88: 557-560.

Bremer G. Problems in the breeding and cytology of sugar cane. Volume II. Sugarcane breeding from a cytological view-point. Euphytica 1961; 10: 121-33.

Burner D. Chromosome transmission and meiotic behavior in various sugarcane crosses. J Amer Soc Sugar Cane Technol 1997; 17: 38-50.

Cuadrado A, Acevedo R, Espina SMD, Jouve N, Torre CD. Genome remodeling in three modern S.officinarum x S.spontaneum cultivars. J Exp Bot 2004; 55: 847-54.

d’Hont A, Ison D, Alix K, Roux C, Glaszmann JC. Determination of basic chromosome numbers in the genus Saccharum by physical mapping of ribosomal RNA genes. Genome 1998; 41: 221-5.

Daniels J, Roach BT. Taxonomy and evolution. In: Heinz, DJ, editor. Sugarcane improvement through breeding. New York: Elsevier; 1987. p. 7-84.

De Laat AMM, Godhe W, Vogelzang MJ. Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breed 1987; 99: 303-7.

Dolezel J. Applications of flow cytometry for the study of plant genomes. J Appl Genet 1997; 38: 285-302.

Dolezel J, Binarova P, Lucreti S. Analysis of nuclear DNA content in plant cells by flow cytometry. Biol Planta 1989; 31:113-20.

Francis DM, Davies S, Barlow PW. A Strong Nucleotypic Effect on the Cell Cycle Regardless of Ploidy Level. Ann Bot 2008; 101: 747-57.

Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E. Rapid flow cytometric analysis of the cell cycle in intact plant- tissues. Science 1983; 220: 1049–51.

Hammat N, Blackwall NW, Davey MR. Variation in the DNA content of Glycine species. J Exp Bot 1991; 42: 659-65.

Ho I, Rayburn AL. The relationship between chloroplast number and genome number in Zea mays spp. Plant Sci 1991; 74: 255–60.

Huff DR, Palazzo AJ. Fine fescue species determination by flow cytometry. Crop Sci 1998; 38: 445-50.

Johnson PG, Riordan TP, Arumuganathan K. Ploidy level determinations in buffalograss clones and populations. Crop Sci 1998; 38: 478-82.

Knight CA, Beaulieu JM. Genome size scaling through phenotype space. Ann Bot 2008; 6: 759-66.

Knight CA, Molinari NA, Petrov DA. The large genome constraint hypothesis: evolution, ecology, and phenotype. Ann Bot 2005; 95: 177-90.

Lam E, Shine J Jr, Da SJ, Lawton M, Bonos S, Calvino M, Carrer H, Silva-Filho MC, Glynn N, Helsel Z, Ma J, Richard F Jr, Souza GM. Improving sugarcane for biofuel: engineering for an even better feedstock. Glob change Biol bioenergy 2009; 1: 251-5.

McCormick AJ, Watt DA, Cramer MD. Supply and demand: sink regulation of sugar accumulation in sugarcane. J Exp Bot 2009; 60: 357-64.

Nair MK. Cytogenetics of Saccharum officinarum L. and S. spontaneum L. IV. Chromosome number and meiosis in S. officinarum x S. spontaneum hybrids. Caryologia 1975; 28: 1-14.

Palomino G, Dolezel J, Mendez I, Rubluo A. Nuclear genome size analysis of Agave tequiliana Weber. Caryologia 2003; 56: 37-46.

Price S. Cytological studies in Saccharum and allied genera. VI. Chromosome numbers in S. officinarum and other noble sugar canes. Hawaii Plant Rec 1960; 56: 183-94.

Rayburn AL, Biradar DP, Bullock DG, McMurphy LM. Nuclear DNA content in F1 hybrids of maize. Heredity 1993; 70: 294-300.

Roach BT. Cytological studies in Saccharum. Chromosome transmission in interspecific and intergeneric crosses. Proc Int Soc Sugar Cane Technol 1969; 13: 901-20.

Soltis DE, Soltis PS. Molecular data and the dynamic nature of polyploidy. Crit Rev Plant Sci 1993; 12: 243-73.

Sreenivasan T, Ahloowalia BS, Heinz DJ. Cytogenetics. In: Heinz, DJ, editor. Sugarcane improvement through breeding. New York: Elsevier; 1987. p. 211-53.

Suda J, Kyncl T, Freiova R. Nuclear DNA amounts in Macaronesian angiosperms. Ann Bot 2003; 92(1): 153-64.

Published

23-05-2017

How to Cite

S.K. Ghosal, A. M., & Kalyan Kumar De, T. P. (2017). Determination of DNA content and relative 2C genome sizes of some promising commercial varieties of sugarcane using flow cytometer. Current Botany, 8. https://doi.org/10.19071/cb.2017.v8.3207

Issue

Section

Regular Articles