Spectrophotometric Method Development and Validation for Estimation of Linezolid in Tablet Dosage Form

Abstract

Linezolid is oxazolidinone antibiotic. It inhibits bacterial protein synthesis by acting at an early step and a site different from that of other Antimicrobial agents. The aim of the present research work was to develop accurate and rapid Differential spectrophotometric (Method A) and Area under Curve (Method B) to determine Linezolid content in pharmaceutical formulation. Differential spectrophotometric method is simplest and rapid technique for altering the spectral properties of analyte with the adjustment of the pH by means of aqueous solutions of acid, alkali or buffers. Differential spectrophotometry based on the principle of ionization of functional groups and pH of the solution. The difference absorption spectra of equimolar solution of Linezolid in 0.1M hydrochloric acid in sample cell relative to 0.1M sodium hydroxide in reference cell were taken. Area under curve method involves the calculation of integrated value of absorbance with respect to the wavelength between the two selected wavelengths. Absorption maxima was found 258.27 nm (Method A) and area under curve was measured between253-263nm.The drug followed a linear relationship in the range of 4-20µg/ml; while the correlation coefficient was 0.999(Method A) and 0.9993 (Method B). The recovery was 100.01% ±0.17 (Method A) and 99.95 ± 0.33, the relative standard deviation for repeatability, intraday and interday was found to be less than 2%. These methods are found suitable for day to day analysis of linezolid in tablet dosage form.