Synthesis of antimicrobial silver nanoparticles by callus cultures and in vitro derived plants of Catharanthus roseus

Abstract

This study highlights the development of a biological method to synthesize silver nanoparticles using the aqueous leaf, callus, and root extracts of in vitro derived plants of Catharanthus roseus. The antibacterial activity of synthesized silver nanoparticles was assessed against different clinical samples such as Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Candida albicans. The highest antibacterial activity was noticed with stabilized silver nanoparticles against all the tested pathogens. Silver nanoparticles synthesized in this process are quiet fast and low cost. Therefore, current study justified the development of eco-friendly process for the synthesis of silver nanoparticles using plant extracts which is an important step in the field of application of nanotechnology. This green chemistry has many advantages such as, quick production, cost effective and easy way to produce nanoparticles.