Optimization of Physico-Chemical Parameters for the Extraction of Phenolic Components from Terminalia chebula Species

Abstract

Terminalia chebula is a moderate tree used in traditional medicines. It contains Chebulinic acid, Quercetin, Tannic acid and many other compounds. The objectives of this work were extraction of phenolic compounds like Total Phenolic Content, Chebulinic acid and Quercetin. The present studies on optimization of physico-chemical parameters like effects of different solvents, soaking time, extraction time with hexane, particle size, different solvent percentages, different volumes of hexane with ethanol and methanol as solvents and pH for the extraction of Total Phenolic Content, Chebulinic acid and Quercetin were studied. For the extraction of Total Phenolic Content, the optimum results were observed for the effects of different solvents, soaking time, extraction time with hexane, particle size, different solvent percentages, different volumes of hexane with ethanol as solvent and pH were ethanol, 1 day, 1hrs, 125 microns, 50% (v/v), 1:1 ratio and 7.0 respectively. The highest Total Phenolic Content concentration for optimized conditions was 2.25mg/dl. For the extraction of Chebulinic acid, the optimum results were observed for the effects of different solvents, soaking time, extraction time with hexane, particle size, different solvent percentages, different volumes of hexane with ethanol as solvent and pH were ethanol, 1 day, 1hrs, 125 microns, 50% (v/v), 1:1 ratio and 7.0 respectively. The highest Total Phenolic Content concentration for optimized conditions was 3.4mg/ml. For the extraction of Quercetin, the optimum results were observed for the effects of different solvents, soaking time, extraction time with hexane, particle size, different solvent percentages, different volumes of hexane with methanol as solvent and pH were methanol, 2 days, 1hrs, 125 microns, 80% (v/v), 1:1 ratio and 6.0 respectively. The highest Quercetin concentration for optimized conditions was 0.54mg/cl.