In vitro Evaluation of Antibacterial Efficacy using Passiflora foetida Activated Carbon

Abstract

Activated carbon (AC) has found its attention in pollution control and wastewater treatment to remove various pollutants. Activated carbon can be prepared by physical and chemical activation methods. The chemical activation methods were advantageous over physical activation due to process accomplishment at lower temperature and greater yield which avoids burn-off char. Activated carbon was usually used as an adsorbent for dye removal from wastewater which could be related to their extended surface area, high adsorption capacity, microporous structure and special surface reactivity. Microbial pollution and contamination have produced various problems in industrial and medical fields. Based on this, the present study was attempted on the preparation of activated carbon from Passiflora foetida and evaluating for its antibacterial efficacy against twelve different microorganisms by agar well diffusion method. The results were found to be very effective with higher zone of inhibition against almost all the microorganisms tested. Moreover, the activity was considerably more against Shigella flexneri (MTCC 1457) and Klebsiella pneumoniae (MTCC 10309).In addition to this, the shake flask test in saline had proved that the colonial growth of E.coli was inhibited with respect to the concentration of activated carbon and time of incubation.