In vivo study of antimutagenic and antioxidant activity of Glycyrrihza glabra root extract

Abstract

The studying was aimed to inhibition the mutagenecity and oxidation of genomic DNA by use methanol-water extract of Glycyrrihza glabra root (GL) against anticancer drug cyclophosphomide in rats by use DNA fragmentation test, the extract characterized by thin layer chromatography TLC, and antioxidant activity by use β- carotene spray, the experiment design to use tow single dose of GL and single dose of the drug in three interaction, extract before, with and after the drug to investigate the mechanism of which extract effect inside cell. Four multiple doses for drug and extract to investigate the accumulation effect in DNA the results show that extract is contain of different polar compound and have antioxidant activity, so the extract is bio-antimutagenesis and poor dismutagenesis activity, the extract can inhibit accumulation effect of mutagenecity and oxidation activity of the drug.