An invitro antimicrobial activity and Bioactivities of Protein Isolated from Marine Sponge – Callyspongia sp.

Abstract

The marine sponge Callyspongia sp, collected from Mandapam coast, Tamilnadu, was studied for antibacterial and antifungal activity of proteins. Sponge species were identified based on spicules morphology. Chloroform and aqueous extracts of Callyspongia sp yielded a total amount of 4.8g and 5.98g from 500g of sponge respectively. Crude protein obtained from marine sponge aqueous extract was 2.1 mg/ml and that of chloroform extract was 0.9 mg/ml. The antimicrobial activity of chloroform extract and aqueous extract showed clear inhibition zone against Pseudomonas sp. Vibrio cholerae, Aspergillus niger and Candida albicans. Both the extracts exhibited hemolytic activity which was estimated as 10.1ht/ml for chloroform extract and 8.6ht/ml for aqueous extract. The partial purification of protein was carried out using DEAE cellulose. On SDS-PAGE the crude protein yielded three well defined bands at 109.9, 28.2, 12.4 KDa respectively by both the extracts.