In vitro cytotoxic activity of methanolic extract of Cardiospermum canescens Wall. (Sapindaceae)

Abstract

The present study was designed to evaluate the in vitro cytotoxicity activity of methanolic extract of Cardiospermum canescens. In this study the extract was tested using human cancer cell lines, Human ductal breast cancer cell line and Colon cancer cell line for their effects on cell viability, growth inhibition and cell morphology. Cell viability and inhibition were determined by MTT [(3,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)] assay by using DAPI (4- 6-diamidino- 2- phenylindole) staining technique. The significant decrease in cell viability was observed for 100µg/ml in human ductal breast cancer cell line and 150µg/ml in colon cancer cell line. The IC50 value is 31.25µg/ml and 250µg/ml in ductal breast and colon cancer cell lines respectively. The results indicated that methanolic extract of Cardiospermum canescens has a potential cytotoxicity activity on Human ductal breast cancer cell line than the colon cancer cell lines.