Design, Development and Evaluation of Dendritic Architechture for the Delivery of Ofloxacin against Resistance Producing Strains

Abstract

Dendrimer represents hyperbranched, monodisperse, three-dimensional macromolecules with host-guest entrapment properties having defined molecular weight. Dendrimer allows defined control of size, shape and position of functional groups. For the above said reason, dendrimers have become more popular application in many fields.The present study was aimed at developing and exploring the use of novel PEGylated 0.5G EDA-PPI (poly propyeleneimine) dendritic architecture for the delivery of an antibacterial drug, Ofloxacin. The dendritic architecture was synthesized by double Michael addiction reaction using ethylenediamine as a core moiety and the synthesized system was PEGylated using PEG-4000 and the Ofloxacin was loaded by equilibrium dialysis method into the system. The prepared Ofloxacin loaded PEGylated 0.5G EDAPPI dendritic architecture was evaluated for FTIR studies, solubility studies, drug entrapment efficiency, in-vitro drug release studies and anti-bacterial assay. The results showed that there was enhanced rate of drug release, drug solubility with significant antimicrobial activity compared to plain Ofloxacin. The tablets of Ofloxacin loaded PEGylated 0.5G EDA-PPI dendrimer were prepared by direct compression method and evaluated for various parameters such as hardness, thickness, weight variation, drug content and in-vitro drug release studies. The in-vitro drug release of Ofloxacin loaded 0.5G EDA-PPI dendrimer compared with that of marketed Ofloxacin tablet. The results revealed that there is enhanced rate of dissolution of Ofloxacin loaded PEGylated 0.5G EDA-PPI dendrimer than that of marketed Ofloxacin tablet formulation.