Quorum sensing and Biofilm formation by Bacterial Isolates from Hemodialysis Patients

Abstract

In this study, blood and urine samples were collected from (60) patients with hemodialysis with age range between (12-82) years old from both sexes. All samples were subjected to identify the bacterial aerobic cultivation. Different types of bacteria were isolated that caused septicemia or urinary tract infection. The predominant bacteria were E. coli for Gram negative, and S. aureus for gram positive. Quorum sensing were studied for E. coli and K. pneumoniae and the results shown that all tested bacteria show an aggregation of the bacterial cells in the presence of homoserine lactone and the best interval for accumulation of homoserine lactone was after 4 hours in E. coli, 2 hours in K. pneumoniae. biofilm formation were studied for all bacterial isolates and the results shown that the all bacteria can form biofilm, S. epidermidis (87.5%), S. aureus (85.7%) as strong where E. coli and Proteus mirabilis show moderate biofilm formation.