Effect of some factors on extracellular hemolysin filtrate from bacterial Pseudomonas aeruginosa isolated from burn infection in Hilla city

Abstract

A total of (50) samples were collected from burn unit of Hilla Teaching Hospital, isolated from patient who suffering from burn cases only (40) (80%) samples were positive culture while other was negative culture (10) (20%), so, a total of (40) samples were isolated; these samples were subjected on different types of media to isolated Pseudomonas aeruginosa. Out of (40) samples, only (15) (37.5%) isolated were belonged to Pseudomonas aeruginosa. the ability of Pseudomonas aeruginosa isolated from patients with burn infection to produced capsule was studied; it was fond that all these isolated (100%) have capsules surrounding bacterial cell, and all isolated have not ability to produce sidrophores enzymes. Also it was studied the group of enzymes produced from bacteria, it was found that all these isolated have ability to produce lipase, protease, urease and bacitracin enzymes. On the other hand, the hemolysin production by Pseudomonas aeruginosa was studied and it was found that all these isolates of these bacteria were able to produce hemolysin on blood agar. Also, extracellular hemolysin filtrates of P. aeruginosa was detected on blood agar, it was found that (10) (66.6%) isolated of theses bacteria have extracellular hemolysin filtrates, it was appearance as transparent area around the well filled with hemolysin filtrates after incubation for 24 hours. The effect of H2O2 on extracellular hemolysin filtrate of P. aeruginosa was studied, and it was found that the H2O2 was decrease the activity of extracellular hemolysin filtrate on blood agar. So, the effect of mannitol and glycerol on hemolysin filtrate produce by P. aeruginosa was studied, and it was found that all isolates of hemolysin filtrate will increased activity when added mannitol and glycerol to it. Finally, the effect of phospholipase C on hemolysin filtrate produce by P. aeruginosa was studied, and it was found that all isolates of hemolysin filtrate will increase activity when added phospholipase C to it.