Molecular phylogenetic analysis of Enterobacter spp. isolated from urine of patients with cystitis in Babylon province, Iraq

Authors

  • Mohammad S. Abdul-Razzaq, Hussein O. Al- Dahmoshi and Ilham A. Bunyan

Abstract

Enterobacter species are important nosocomial pathogens responsible for various extraintestinal and intestinal infections. Both Enterobacter cloacae and Enterobacter aerogenes can cause hospital acquired and community acquired urinary tract infections. The aim of this study was to determine the phylogenetic analysis of Enterobacter spp. isolates. A total of 24 Enterobacter local isolates (9 isolates were E. cloacae (EC) and 15 isolates were E. aerogenes (EA) recovered from urine samples of patients with cystitis and subjected for PCR to determine the phylogenetic groups and subgroups by targeting two gene chuA, yjaA and anonymous DNA fragment TspE4.C2.The results found that the most isolates of Enterobacter spp. belong to the phylogeny group B2, 17(70.84%) and the largest subgroups was B23 (12/17) followed by subgroup B22 (5/17). The second phylogenetic group was group A, 4(16.66%) in which subgroup A0 compile (3/4) and A1(1/4). Group B1 compile 2(8.34%) followed by group D 1(4.16%). This study was the first to determine the phylogenetic groups of Enterobacter spp. isolates and demonstrate that these bacteria can be assigned to one of the main phylogenetic groups. Our results revealed that, Phylogenetic group B2(especially subgroup B23) was predominant among Enterobacter spp. isolates recovered from patients with cystitis.

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Published

29-10-2015

How to Cite

Dahmoshi and Ilham A. Bunyan, M. S. A.-R. H. O. A.-. “Molecular Phylogenetic Analysis of Enterobacter Spp. Isolated from Urine of Patients With Cystitis in Babylon Province, Iraq”. Research in Pharmacy, vol. 3, no. 1, Oct. 2015, https://updatepublishing.com/journal/index.php/rip/article/view/225.

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