Identification of seed storage protein markers for drought tolerance in mungbean

Authors

  • Swapan Kumar Tripathy Orissa University of Agriculture & Technology
  • Priyadarshini Mohanty Res. Scholar, Dept. of Plant Breed. & Gewnet.,CA, OUAT, Bhubaneswar
  • Monalisha Jena M.Sc.(AG.) student, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar
  • Sasmita Dash Asst. Training instructor, OUAT polytechnic Centre, Ranital, OUAT, Odisha
  • Devraj Lenka Res. Scholar, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar, Odisha
  • Dayanidhi Mishra Pl. Breeder, AICRP(Potential Crops), OUAT, Bhubaneswar, Odisha
  • Pramod Kumar Nayak Plant Breeder, AICRP(Sugarcane), Nayagarh, OUAT, Odisha
  • Digbijaya Swain Plant Breeder, AICRP(Maize), OUAT, Bhubaneswar
  • Rajesh Ranjan Res. Scholar, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar, Odisha
  • Kedareswar Pradhan Professor, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar, Odisha
  • Niranjan Senapati Assoc. Director of Res., RRTTS, OUAT, Similiguda, Odisha
  • Pavitra Mohan Mohapatra Pl. Breeder, AICRP(Millet), Berhampur, OUAT, Odisha
  • Gokul Bihari Dash Pl. Breeder, AICRP(Forage crops), OUAT, Bhubaneswar, Odisha

DOI:

https://doi.org/10.19071/rib.2016.v7.2895

Abstract

A set of 292 mungbean germplasm accessions including 62 popularly adapted local land races and two wild forms (Vigna radiata var. sublobata), important breeding lines and standard ruling varieties were screened for drought stress tolerance at seedling stage.  Eight genotypes e.g., C. No. 35, OUM 14-1, OUM 49-2, Pusa 9072, OM 99-3, Banapur local B, Nipania munga, Kalamunga 1-A) have been identified to possess drought tolerance.  Globulin seed storage protein profiling was carried out in 19 selected mungbean genotypes comprising eight drought tolerant, seven drought sensitive, two wild forms of mungbean (TCR 20 and TCR 213) and two standard checks (LGG 460 and T 2-1) to explore differentially expressed polypeptides. Seed protein profiles revealed 15 scorable polypeptide bands with molecular weights ranging from 10.0 to 102.2kD. A specific 12.8kD polypeptide band was present in all above drought tolerant test genotypes including the wild accession TCR 20. Such a polypeptide band may serve as useful biochemical marker for identification of drought tolerant genotypes in mungbean.

            Key words: Genetic diversity, seed storage protein profile, wild and cultivated Vigna radiata.

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Author Biographies

Swapan Kumar Tripathy, Orissa University of Agriculture & Technology

Professor(ABT), Department of Agricultural Biotechnology, College of Agriculture, OUAT, Bhubaneswar-751003,Odisha(INDIA)

Priyadarshini Mohanty, Res. Scholar, Dept. of Plant Breed. & Gewnet.,CA, OUAT, Bhubaneswar

Res. Scholar, Dept. of Plant Breed. & Gewnet.,CA, OUAT, Bhubaneswar

Monalisha Jena, M.Sc.(AG.) student, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar

M.Sc.(AG.) student, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar

Sasmita Dash, Asst. Training instructor, OUAT polytechnic Centre, Ranital, OUAT, Odisha

Asst. Training instructor, OUAT polytechnic Centre, Ranital, OUAT, Odisha

Devraj Lenka, Res. Scholar, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar, Odisha

Res. Scholar, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar, Odisha

Dayanidhi Mishra, Pl. Breeder, AICRP(Potential Crops), OUAT, Bhubaneswar, Odisha

Pl. Breeder, AICRP(Potential Crops), OUAT, Bhubaneswar, Odisha

Pramod Kumar Nayak, Plant Breeder, AICRP(Sugarcane), Nayagarh, OUAT, Odisha

Plant Breeder, AICRP(Sugarcane), Nayagarh, OUAT, Odisha

Digbijaya Swain, Plant Breeder, AICRP(Maize), OUAT, Bhubaneswar

Plant Breeder, AICRP(Maize), OUAT, Bhubaneswar

Rajesh Ranjan, Res. Scholar, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar, Odisha

Res. Scholar, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar, Odisha

Kedareswar Pradhan, Professor, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar, Odisha

Professor, Dept. of Pl. Breed. & Genet., CA, OUAT, Bhubaneswar, Odisha

Niranjan Senapati, Assoc. Director of Res., RRTTS, OUAT, Similiguda, Odisha

Assoc. Director of Res., RRTTS, OUAT, Similiguda, Odisha

Pavitra Mohan Mohapatra, Pl. Breeder, AICRP(Millet), Berhampur, OUAT, Odisha

Pl. Breeder, AICRP(Millet), Berhampur, OUAT, Odisha

Gokul Bihari Dash, Pl. Breeder, AICRP(Forage crops), OUAT, Bhubaneswar, Odisha

Pl. Breeder, AICRP(Forage crops), OUAT, Bhubaneswar, Odisha

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Published

23-03-2016

How to Cite

Tripathy, S. K., Mohanty, P., Jena, M., Dash, S., Lenka, D., Mishra, D., Nayak, P. K., Swain, D., Ranjan, R., Pradhan, K., Senapati, N., Mohapatra, P. M., & Dash, G. B. (2016). Identification of seed storage protein markers for drought tolerance in mungbean. Research in Biotechnology, 7. https://doi.org/10.19071/rib.2016.v7.2895

Issue

Vol. 7 (2016)

Section

Research Articles

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