Revealing contrasting genetic variation and study of genetic diversity in urdbean (Vigna mungo (L.) Hepper) using SDS-PAGE of seed storage proteins

Authors

  • Swapan Kumar Tripathy Orissa University of Agriculture & Technology
  • Priyadarshini Mohanty Res. Scholar, Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar
  • Monalisha Jena M.Sc.(Ag.) student, Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar
  • Gokul Bihari Dash Plant Breeder, AICRP(Forage crops), OUAT, Bhubaneswar
  • Kedareswar Pradhan Professor(PB&G), Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar
  • Pramod Kumar Nayak Plant Breeder, AICRP(Sugarcane), SRS, Nayagarh, OUAT, Odisha
  • Sasmita Dash Training Instructor, Agro-polytechnic, OUATRanital, Bhadrak
  • Devraj Lenka Assoc. Professor(PB&G), Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar
  • Dayanidhi Mishra Plant Breeder, AICRP(Potential Crops), OUAT, Bhubaneswar
  • Pavitra Mohan Mohapatra Plant Breeder, AICRP(Millets),OUAT, Berhampur, Ganjam, Odisha
  • Digbijaya Swain Plant Breeder, AICRP(Maize), OUAT, Bhubaneswar, India
  • Niranjan Senapati Associate Director of Research, RRTTS, Similiguda, OUAT, Odisha, INDIA

DOI:

https://doi.org/10.19071/rib.2016.v7.2863

Abstract

Total seed storage protein profiles of 20 urdbean genotypes including the popular variety T9 were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 14 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into three protein types. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 78.5% phenon level.  TU 95-1 with TU 12-25-4 revealed lowest similarity index value (0.33) followed by TU 95-1 with PU 30 and KU 96-3(SI=0.35). Clustering pattern revealed distinctly divergent group formed by TPU 95-1 and TPU 4. These may serve as a valuable source genotype in recombination breeding.   

Key words: Seed storage protein profiling, SDS-PAGE, Genetic variation, urdbean.

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Author Biographies

Swapan Kumar Tripathy, Orissa University of Agriculture & Technology

Professor(ABT), Department of Agricultural Biotechnology, College of Agriculture, OUAT, Bhubaneswar-751003,Odisha(INDIA)

Priyadarshini Mohanty, Res. Scholar, Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar

Res. Scholar, Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar

Monalisha Jena, M.Sc.(Ag.) student, Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar

M.Sc.(Ag.) student, Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar

Gokul Bihari Dash, Plant Breeder, AICRP(Forage crops), OUAT, Bhubaneswar

Plant Breeder, AICRP(Forage crops), OUAT, Bhubaneswar

Kedareswar Pradhan, Professor(PB&G), Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar

Professor(PB&G), Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar

Pramod Kumar Nayak, Plant Breeder, AICRP(Sugarcane), SRS, Nayagarh, OUAT, Odisha

Plant Breeder, AICRP(Sugarcane), SRS, Nayagarh, OUAT, Odisha

Sasmita Dash, Training Instructor, Agro-polytechnic, OUATRanital, Bhadrak

Training Instructor, Agro-polytechnic, OUATRanital, Bhadrak

Devraj Lenka, Assoc. Professor(PB&G), Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar

Assoc. Professor(PB&G), Dept. of Pl. Breed. & Genetics, CA, OUAT, Bhubaneswar

Dayanidhi Mishra, Plant Breeder, AICRP(Potential Crops), OUAT, Bhubaneswar

Plant Breeder, AICRP(Potential Crops), OUAT, Bhubaneswar

Pavitra Mohan Mohapatra, Plant Breeder, AICRP(Millets),OUAT, Berhampur, Ganjam, Odisha

Plant Breeder, AICRP(Millets),OUAT, Berhampur, Ganjam, Odisha

Digbijaya Swain, Plant Breeder, AICRP(Maize), OUAT, Bhubaneswar, India

Plant Breeder, AICRP(Maize), OUAT, Bhubaneswar, India

Niranjan Senapati, Associate Director of Research, RRTTS, Similiguda, OUAT, Odisha, INDIA

Associate Director of Research, RRTTS, Similiguda, OUAT, Odisha, INDIA

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Published

06-02-2016

How to Cite

Tripathy, S. K., Mohanty, P., Jena, M., Dash, G. B., Pradhan, K., Nayak, P. K., Dash, S., Lenka, D., Mishra, D., Mohapatra, P. M., Swain, D., & Senapati, N. (2016). Revealing contrasting genetic variation and study of genetic diversity in urdbean (Vigna mungo (L.) Hepper) using SDS-PAGE of seed storage proteins. Research in Biotechnology, 7. https://doi.org/10.19071/rib.2016.v7.2863

Issue

Vol. 7 (2016)

Section

Research Articles

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