Effect of media and gelling agents on shoot organogenesis of Liriope platyphylla

Authors

  • Young Chang Kim Research Policy Bureau, Convergence and Innovation Strategy Team, 300 Nongsaengmyeong-ro, Deokjingu, Jeonju-si, Jellabuk-do 54875, Republic of Korea
  • Woo Tae Park Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Republic of Korea
  • Ramaraj Sathasivam Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon 34134, Republic of Korea
  • Haeng-Hoon Kim Department of Agricultural Life Science, Sunchon National University, Suncheon 57922, Republic of Korea
  • Jae Kwang Kim Division of Life Sciences and Convergence Research Center for Insect Vectors, College of Life Sciences and Bioengineering, Incheon National University, Yeonsu-gu, Incheon 22012, Republic of Korea
  • Sang Un Park Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon 34134, Republic of Korea

DOI:

https://doi.org/10.25081/jp.2023.v15.8319

Keywords:

Gelling agent, Shoot organogenesis, Liriope platyphylla, Plant agar, Gellan gum

Abstract

Liriope platyphylla can be multiplied either by planting seeds or dividing its tuberous roots. In this study, a method for L. platyphylla plant shoot organogenesis from meristem explants was developed employing medium and gelling agents. For the selection of optimal medium conditions for shoot organogenesis, the effects of full- and half-strength B5, SH, and MS media were examined. Different concentrations of the gelling agents such as phytagar (6, 7, 8, and 9 g L-1) and gellan gum (2, 3, 4, and 5 g L-1) were examined for efficient shoot formation. The results revealed the superiority of half-strength MS basal medium in shoot organogenesis and growth of Liriope platyphylla. But the half-strength B5 media performed poorly. Compared to plant agar, gellan gum performed well in terms of shoot regeneration and shoot length. When gellan gum was used at 3 g L-1 the maximum number of shoots explant-1 (5.8) and longest shoot (45.8 mm) was observed but the lowest number of shoots explant-1 (3.2) and shortest shoot (21.4 mm) was registered with 5 g L-1. It is proposed from our study that half-strength MS media and gellan gun gelling agent at 3 g/l could be applied in shoot organogenesis and growth of L. platyphylla.

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Published

20-04-2023

How to Cite

Kim, Y. C., Park, W. T., Sathasivam, R., Kim, H.-H., Kim, J. K., & Park, S. U. (2023). Effect of media and gelling agents on shoot organogenesis of Liriope platyphylla. Journal of Phytology, 15, 52–56. https://doi.org/10.25081/jp.2023.v15.8319

Issue

Volume 15, 2023

Section

Articles

Copyright (c) 2023 Young Chang Kim, Woo Tae Park, Ramaraj Sathasivam, Haeng-Hoon Kim, Jae Kwang Kim, Sang Un Park

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