In vitro cytotoxic potential of Gymnema sylvestre fractions on HT-29 cell line

Authors

  • Shanmugapriya Arumugam Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore – 641 021, Tamil Nadu, India
  • Sangilimuthu Alagar Yadav Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore – 641 021, Tamil Nadu, India
  • Abinaya Krishnan Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore – 641 021, Tamil Nadu, India
  • Sowmya Priya Manoharan Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore – 641 021, Tamil Nadu, India
  • Suvathika Gnanaselvan Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore – 641 021, Tamil Nadu, India

DOI:

https://doi.org/10.25081/jp.2022.v14.7942

Keywords:

Gymnema sylvestre, Colon cancer, Cytotoxicity, Apoptosis, Cell cycle arrest

Abstract

The HT-29 colon cancer cell line was used in the current work to investigate the anti-cancer efficacy of Gymnema sylvestre extracts. The viability, apoptosis, flow cytometry, and mode of action of the active fraction were also evaluated. The active fractions of G. sylvestre caused cell cycle arrest at the S phase and G2/M phase, and also caused programmed cell death. The alcoholic fractions showed anti-cancer effect in HT-29 cancer cells. By applying methanolic fraction of Gymnema sylvestre to the HT-29 cells, IC50 was observed at 140.39 μg/mL, whereas for chloroform fraction of Gymnema sylvestre showed IC50 at 154.44 μg/mL. The herbal extract for colon cancer treatment may be developed with the help of researchers due to G. sylvestre’s anti-cancer properties.

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Published

18-11-2022

How to Cite

Arumugam, S., Yadav, S. A., Krishnan, A., Manoharan, S. P., & Gnanaselvan, S. (2022). In vitro cytotoxic potential of Gymnema sylvestre fractions on HT-29 cell line. Journal of Phytology, 14, 121–126. https://doi.org/10.25081/jp.2022.v14.7942

Issue

Volume 14, 2022

Section

Articles

Copyright (c) 2022 Shanmugapriya Arumugam, Sangilimuthu Alagar Yadav, Abinaya Krishnan, Sowmya Priya Manoharan, Suvathika Gnanaselvan

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