Integrated analysis of Tinospora cordifolia germplasm: Nutritional, qualitative, quantitative, and phytochemical traits: Integrated analysis of Tinospora

B C Akhilraj; J Suresh; K Rajamani; M Kumar; R Gnanam

doi:10.25081/josac.2024.v33.i2.9542

Authors

B C Akhilraj Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu
J Suresh Horticultural College and Research Institute for Women, Tamil Nadu Agricultural University, Aliyar Nagar, Pollachi, Tamil Nadu
K Rajamani Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu
M Kumar ICAR- Krishi Vigyan Kendra, Tamil Nadu Agricultural University, Tindivanam, Villupuram, Tamil Nadu
R Gnanam Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu

DOI:

https://doi.org/10.25081/josac.2024.v33.i2.9542

Keywords:

Germplasm, Tinospora cordifolia, Tamil Nadu, qualitative, quantitative

Abstract

Tinospora cordifolia, often known as "Guduchi" is a deciduous climber and is considered valuable in terms of its immense medicinal properties. This study evaluated 48 Tinospora cordifolia germplasm accessions collected from Tamil Nadu for their nutritional, qualitative, quantitative, and phytochemical properties. Significant variations were observed among the accessions. TC-22 exhibited the highest phenol content (122.73 mg/g) and flavonoid content (18.84 mg/g), while TC-30 recorded the highest tannin content (234.56 mg/g). TC-20 demonstrated superior protein (13.85%), carbohydrate (86.92 mg/g), and antioxidant activity (261.74%) levels. Additionally, TC-20 displayed elevated potassium (1.45%), calcium (0.35%), and iron (48.74%) levels, with the highest zinc content (15.67 μg/g) among accessions. TC-30 had the highest fat content (8.81%), while TC-22 recorded the maximum cardiac glycosides (2.27%). The study identified TC-35 as having the highest fiber content (13.22%) and energy value (99.66 Kcal/100g). Vitamin C (68.96 mg/100g) and vitamin A (0.44 mg/100g) were most abundant in TC-33. These findings provide valuable insights into the biochemical diversity of T. cordifolia germplasm, highlighting its potential for nutritional and therapeutic applications. Findings unmistakably demonstrated the significance of ecological condition variables in determining the quantitative and qualitative parameters of diverse accessions from the different places of Tamil Nadu.

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References

Ahmed S M, Manhas L R, Verma V & Khajuria R K 2006 Quantitative determination of four constituents of Tinospora sps. by a reversed-phase HPLC-UV-DAD method. Broad-based studies revealing variation in content of four secondary metabolites in the plant from different eco-geographical regions of India. J. Chromatogr. Sci.. 44(8): 504-509.

AOAC 1990 Official Methods of Analysis, 15th edn. Association of Official Analytical Chemists, Arlington, VA

Aranha I, Clement F & Venkatesh YP 2012 Immunostimulatory properties of the major protein from the stem of the Ayurvedic medicinal herb, guduchi (Tinospora cordifolia). J. Ethnopharmacol. 139(2): 366-372.

Armstrong M J, & Kirkby E A 1979 Estimation of potassium recirculation in tomato plants by comparison of the rates of potassium and calcium accumulation in the tops with their fluxes in the xylem stream. Plant Physiol. 63 (6): 1143- 1148.

Bhandal I S & Malik C P 1988 Potassium estimation, uptake, and its role in the physiology and metabolism of flowering plants. In International review of cytology (Vol. 110, pp. 205-254). Academic Press.

Bradford M M 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72(1-2): 248-254.

David D J 1958 Determination of zinc and other elements in plants by atomic-absorption spectroscopy. Analyst. 83(993): 655-661.

Energy F F 2003 Methods of analysis and conversion factors food and paper nutrition paper. Food and Agriculture Organization: Rome, Italy.

Gahlaut A, Gothwal A & Dabur R 2012 TLC based analysis of allelopathic effects on tinosporoside contents in Tinospora cordifolia. J. Chem. Pharm. Res. 4: 3082-3088.

Garg A N, Singh R, Maharia R S, Dutta R K & Datta A 2022 Quantification of minor, trace and toxic elements in stems of Santalum album (L.), Mangiferra indica (L.) and Tinospora cordifolia by instrumental neutron activation analysis. J. Plant Sci. Phytopathol. 6: 8-14.

Gupta R & Sharma V 2011 Ameliorative effects of Tinospora cordifolia root extract on histopathological and biochemical changes induced by aflatoxin-B1 in mice kidney. Toxicol. Int. 18(2): 94-98.

Hassan A S & Hassan H S 2008 Quantitative estimation of vitamin C in some local fruits. Sci. World J., 3(2): DOI:10.4314/swj.v3i2.51805

Chang C C, Yang M H, Wen H M & Chern J C 2002 Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal. 10(3): 178-182

Choudhry N, Singh S, Siddiqui M B & Khatoon S 2014 Impact of seasons and dioecy on therapeutic phytoconstituents of Tinospora cordifolia, a Rasayana drug. Biomed Res. Int., 2014:1-11.

Ingberman A K 1958 Functionality of Phenols by Bromination. Anal. Chem., 30(5): 1003-1004.

Jones Jr J B 1991 Kjeldahl method for nitrogen determination. Micro-Macro Publishing, Inc.

Khan I M, Sri Harsha P S C , Giridhar P & Ravishankar G A 2011 Pigment identification, antioxidant activity, and nutrient composition of Tinospora cordifolia (willd.) Miers ex Hook. f & Thoms fruit. Int. J. Food Sci. Nutr. 62(3): 239-249.

Khan T A, Ipshita A H, Mazumdar R M, Abdullah A T M, Islam G M R & Rahman M M 2020 Bioactive polyphenol profiling and in-vitro antioxidant activity of Tinospora cordifolia Miers ex Hook F and Thoms: a potential ingredient for functional food development. Bangladesh J. Sci. Ind. Res. 55(1): 23-34.

NMPB https://www.nmpb.nic.in. Accessed 03 December 2024

Kirk P L 1950 Kjeldahl method for total nitrogen. Anal. chem. 22(2): 354-358.

Koleva I I, Van Beek T A, Linssen J P, Groot A D & Evstatieva L N 2002 Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal. 13(1): 8-17.

Kumar A, Kumar M, Dandapat S & Sinha M P 2013 Antioxidant activity and pharmacological screening of Tinospora cordifolia (Thunb.). The Bioscan. 8(2): 689-693

Lopez-Teros V, Ford J L, Green M H, Tang G, Grusak M A, Quihui-Cota L, & Astiazaran-Garcia H 2017 Use of a “super-child” approach to assess the vitamin A equivalence of Moringa oleifera leaves, develop a compartmental model for vitamin A kinetics, and estimate vitamin A total body stores in young Mexican children. J. Nutr. 147(12): 2356-2363.

Majed N, Li Y & Gu A Z 2012 Advances in techniques for phosphorus analysis in biological sources. Curr. Opin. Biotechnol. 23(6): 852-859.

McDonald S, Prenzler P D, Antolovich M & Robards K 2001 Phenolic content and antioxidant activity of olive extracts Food Chem. 73(1): 73-84.

Modi B, Kumari Shah K, Shrestha J, Shrestha P, Basnet A, Tiwari I & Prasad Aryal S 2020 Morphology, Biological Activity, Chemical Composition, and Medicinal Value of Tinospora cordifolia (willd.) Miers. Adv. J. Chem. B 3(1): 36-53.

Obadoni B O & Ochuko P O 2002 Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in Edo and Delta States of Nigeria. Global J. Pure Appl. Sci. (2): 203-208.

Premanath R & Lakshmidevi N 2010 Studies on anti-oxidant activity of Tinospora cordifolia (Miers.) leaves using in vitro models. Am. J. Sci. 6(10): 736-743.

Puratchimani V & Jha S 2007 HPTLC standardization of Tinospora cordifolia using tinosporaside. Indian J. Pharm. Sci. 69(4): 578-581.

Sahrawat K L 1987 Determination of calcium, magnesium, zinc and manganese in plant tissue using a dilute HCl extraction method 1. Commun. Soil Sci. Plant Anal. 18(9): 947-962.

Shervani Z A & Mishra P K 2017 Phytochemical study of Tinospora cordifolia grown on three different soil conditions. Res. J. Life Sci. Bioinform. Pharm. Chem. Sci. 5: 810-815.

Shivraj H & Khobragade C N N 2009 Determination of nutritive value and mineral elements of some important medicinal plants from western part of India. J. Med. Plants. 8(29): 79-88.

Shukla S & Gardner J 2006 Local knowledge in community-based approaches to medicinal plant conservation: lessons from India. J. Ethnobiol. Ethnomed. 2: 1-9.

Solich P, Sedliakova V & Karlicek R 1992 Spectrophotometric determination of cardiac glycosides by flow-injection analysis. Analytica. Chimica. Acta. 269(2): 199-203.

Srinivasan G V, Unnikrishnan K P, Shree A R & Balachandran I 2008 HPLC estimation of berberine in Tinospora cordifolia and Tinospora sinensis. Indian J. Pharm. Sci. 70(1): 96-99.

Stien L H, Kiessling A & Manne F 2007 Rapid estimation of fat content in salmon fillets by colour image analysis. J. Food Compos. Anal. 20(2): 73-79.

Talbot G H, Bradley J, Edwards J E, Gilbert D, Scheld M & Bartlett J G 2006 Erratum: Bad bugs need drugs: An update on the development pipeline from the Antimicrobial Availability Task Force of the Infectious Diseases Society of America (Clinical Infectious Diseases (March 1, 2006) 42 (657-668)). Clin. Infect. Dis. 42(7): 1065.

Tanwar S I, Jain J, Verma S & Solanki D 2012 Standardization and phytochemical evaluation of Tinospora cordifolia (willd.) Miers.(Menispermaceae). Int. J. Pharm. Pharm. Sci. 4: 219-223.

Thomas W & Dutcher R A 1924 The colorimetric determination of carbohydrates in plants by the picric acid reduction method 1. The estimation of reducing sugars and sucrose 1. J. Am. Chem. Soc. 46(7): 1662-1669.

Tinggi U, Reilly C & Patterson C 1997 Determination of manganese and chromium in foods by atomic absorption spectrometry after wet digestion. Food Chem. 60(1): 123-128.

Tyagi P & Chauhan A K 2020 Optimization and characterization of functional cookies with addition of Tinospora cordifolia as a source of bioactive phenolic antioxidants. LWT, 130: 109639.

Uadia N R, Ugwu I, Erameh T & Osunde E 2017 Estimation of tannins, alkaloids, saponins and proximate composition of Vernonia amygdalina (Del) root. Int. J. Herb. Med. 5(3): 88-92.

Upadhyay N, Ganie S A, Agnihotri R K & Sharma R 2014 Free radical scavenging activity of Tinospora cordifolia (Willd.) Miers. J. Pharmacogn. Phytochem. 3(2): 63-69.

Van Soest P J & McQueen R W 1973 The chemistry and estimation of fibre. Proc. Nutr. Soc. 32(3): 123-130.

Yadav R N S & Agarwala M 2011 Phytochemical analysis of some medicinal plants. J. Phytol. 3(12): 10-14

Yemm E W & Willis A 1954 The estimation of carbohydrates in plant extracts by anthrone. Biochem. J. 57(3): 508-514.

Integrated analysis of Tinospora cordifolia germplasm: Nutritional, qualitative, quantitative, and phytochemical traits