Metal ion and pH Stable Protease Production Using Agro-industrial Waste

Authors

  • Rajshree Saxena1 and Rajni Singh2*

Abstract

Protease from a newly isolated Bacillus strain exhibited stability in presence of many monovalent and divalent ions and a wide pH range. Agro-industrial waste as Wheat Bran, Gram Husk, Rice Bran and Mustard Oil Cake were used for the production of the enzyme in Solid state fermentation. Maximum enzyme production was observed with rice bran at moisture content 1:3 (w/v) and 20% inoculum size (v/w). The optimum temperature and pH of the enzyme activity were found to be 60oC and 8 respectively. It was found to be stable at 30-50°C for 2 h and pH range of 6-9. The enzyme activity enhanced in presence of  metal ions as Na+, K+, Ca++, Mg++ Cu++, Mn++, Zn++ and Hg++ the activity.

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1Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India

2Amity Institute of Microbial Biotechnology, Amity University, Noida, Uttar Pradesh, India

*Corresponding author, Email: [email protected], Tel: 0120-4392900, Fax: 0120-4392295

Please Cite This Article As:

 

Rajshree Saxena and Rajni Singh. 2010. Metal ion and pH Stable Protease Production Using Agro-industrial Waste. J. Ecobiotechnol. 2(4):01-05.

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Author Biography

Rajshree Saxena1 and Rajni Singh2*

1Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India2Amity Institute of Microbial Biotechnology, Amity University, Noida, Uttar Pradesh, India*Corresponding author, Email: [email protected], Tel: 0120-4392900, Fax: 0120-4392295

Published

29-08-2010

How to Cite

Rajni Singh2*, R. S. and. “Metal Ion and PH Stable Protease Production Using Agro-Industrial Waste”. Journal of Ecobiotechnology, vol. 2, no. 4, Aug. 2010, https://updatepublishing.com/journal/index.php/jebt/article/view/60.

Issue

Section

Industrial Biotechnology