Screening of Biosurfactants from Hydrocarbon Degrading Bacteria

Authors

  • R. Nishanthi1*, S. Kumaran2, P. Palani1, C. Chellaram2, T. Prem Anand2, and V. Kannan1

Abstract

Biosurfactants compounds are produced by microorganisms. These isolates reduced surface tension both in aqueous solutions and hydrocarbon mixtures. The objective of this study was to isolate and identify the biosurfactant producing bacteria from petrochemical soil wastes. Among the 16 isolates, two strains (BPB7: Pseudomonas sp and BPB13: Serratia sp) were shown high salt tolerance and successful production of biosurfactant in a range of pH 6.5 to 7.5 and at room temperature. To confirm the ability of isolates in biosurfactant production, different screening methods including hemolysis, emulsification, penetration, oil spreading test BATH assay and salt aggregation assay were assessed. This study was suggested that these two bacteria having potential for microbial enhanced oil recovery and also possessing Anti microbial and Anti adhesive property. The rich amount of biosurfactant compound like, glycolipid was found in these two strains. (BPB7 and BPB13).

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Author Biography

R. Nishanthi1*, S. Kumaran2, P. Palani1, C. Chellaram2, T. Prem Anand2, and V. Kannan1

1Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai-6000 25, Tamil Nadu, India2Department of Biomedical Engineering, Vel Tech Multi Tech Dr. Rangarajan and Dr. Sakunthala Engineering College,Chennai-600062, Tamil Nadu, India

Published

25-11-2010

How to Cite

C. Chellaram2, T. Prem Anand2, and V. Kannan1, R. N. S. K. P. P. “Screening of Biosurfactants from Hydrocarbon Degrading Bacteria”. Journal of Ecobiotechnology, vol. 2, no. 5, Nov. 2010, https://updatepublishing.com/journal/index.php/jebt/article/view/44.

Issue

Section

Microbial Biotechnology