Agrobacterium mediated Transformation of rice, var. Pusa Basmati-1
Abstract
The present study was aimed at the Agrobacterium mediated transformation of rice (Oryza sativa L.) variety Pusa Basmati-1 .Agrobacterium tumefaciens strain LBA 4404 harboring the binary vector (pIG121) that carries the genes for ß-glucuronidase and hygromycin phosphotransferase (hpt) was used for transformation. Co-cultured calli were undergone for GUS histochemical analysis of transformed calli and followed by molecular analysis by using PCR to confirm the presence of transgenes.
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Published
05-12-2009
How to Cite
M. Kalpana V. Natarajan, R. Panneerselvam, M. A. R. R. “Agrobacterium Mediated Transformation of Rice, Var. Pusa Basmati-1”. Journal of Ecobiotechnology, vol. 1, no. 1, Dec. 2009, https://updatepublishing.com/journal/index.php/jebt/article/view/2.
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REGULAR ISSUE ARTICLES