Expression profiling of stress responsive genes in cell suspension of Elettaria cardamomum (L.) Maton under abiotic stress
DOI:
https://doi.org/10.25081/jpc.2023.v51.i1.8188Abstract
Cardamom is an economically important spice, valued for its multiple utility from culinary to medical purposes. The plant is highly susceptible to both abiotic and biotic stresses. Despite the fact that several studies focusing on stress in cardamom have been conducted; a molecular analysis at the cellular level has not been reported. This study highlights the molecular response of homogenous population of cardamom cell suspension following the temperature and drought stresses for a short period of time (20 mins). Temperature stress at 30, 35, and 40°C caused a significant increase in the transient expression of genes, sHSP 17.8 and sHSP 17.9, which are molecular chaperones involved in protein folding coping with the heat stress response of plants. Drought stress with various concentrations of PEG 6000 has demonstrated only a little increase in the expression of transcription factors, WRKY 35 and WRKY 71. The study implies that sHSP 17.8 and sHSP 17.9 play a crucial role during heat stress, which is a major limiting factor for the cultivation of cardamom in lower altitudes where atmospheric temperature is usually high. But WRKY 35 and WRKY 71 genes are found not to have a high impact at the cellular level in response to drought stress in cardamom when it is exposed to a brief duration of drought. Understanding the molecular mechanism underlying abiotic stress response in cardamom will aid in developing elite varieties adaptable to lower altitudes and to cope with the frequent climatic variations.
Downloads
References
Ashokkumar, K., Murugan, M., Dhanya, M.K. and Warkentin, T.D. 2020. Botany, traditionaluses, phytochemistry and b i o l o g i c a l a c t i v i t i e s o f c a r d a m o m [ Eletta r ia cardamomum (L.) Maton] – A critical review. Journal of Ethnopharmacology 246: 112244.
Chandran, H., Meena, M., BarupChandran, H., Meena, M., Barupal, T. and Sharma, K. 2020. Plant tissue culture as a perpetual source for production of industrially important bioactive compounds. Biotechnology Reports 26.
Chen, X., Lin, S., Liu, Q., Huang, J., Zhang, W., Lin, J., Wang, Y., Ke, Y. and He, H. 2014. Expression and interaction of small heat shock proteins (sHsps) in rice in response to heat stress. Biochimica et Biophysica Acta 1844(4): 818 – 828.
Guy, C. 1999. Molecular responses of plants to cold shock and cold acclimation. Journal of Molecular Microbiology and Biotechnology 1: 231–242.
He, G.H., Xu, J.Y., Wang, Y.X., Liu, J.M., Li, P.S., Chen, M., Ma, Y.Z. and Xu, Z.S. 2016.Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis. BMC Plant Biology 16(1): 1–16.
Kozera, B. and Rapacz, M. 2013. Reference genes in real-time PCR. Journal of Applied Genetics 54(4): 391–406.
Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35(6): 1547–1549.
Li, Z. In Vitro micro-tissue and organ models for toxicity testing. In: Moo-Young M.(eds.),Comprehensive Biotechnology. Academic Press, Burlington, 551–563.
Madhusoodanan, K.J., Kumar, K.P. and Ravindran, P.N. 2002. Botany, crop improvement and biotechnology of cardamom. In: Taylor and Francis (eds.), Cardamom-The genus Elettaria. Taylor & Francis Inc, London, 11 – 68.
Menges, M., HenMenges, M., Hennig, L., Gruissem, W. and Murray, J.A.H. 2003. Genome-wide gene expressionin an Arabidopsis cell suspension. Plant Molecular Biology 53(4): 423–442.
Milioni, D., Franz, G., Sung, R. and Hatzopoulos, P. 2001. Gene expression during heat shock in embryogenic carrot cell lines. Plant Cell, Tissue and Organ Culture 65: 221-228.
Mohammedsani, Z. 2019. Review on breeding method and achievements of cardamom(Elettaria cardamomum Maton) and future prospects. International Journal of Research in Agriculture and Forestry 6(12): 16-23.
Moscatiello, R., Mariani, P., Sanders, D. and Maathuis, F.J.M. 2006. Transcriptional analysis of calcium-dependent and calcium-independent signaling pathways induced by oligogalacturonides. Journal of Experimental Botany 57(11): 2847–2865.
Murakami, T., Matsuba, S., Funatsuki, H., Kawaguchi, K., Saruyama, H., Tanida, M. and Sato, Y. 2004. Over-expression of a small heat shock protein, sHSP17. 7, confers both heat tolerance and UV-B resistance to rice plants. Molecular Breeding 13(2): 165 –175
Murashige, T. and Skoog, F. 1962. Murashige 1962 Revised. Physiologia Plantarum 15: 474–497.
Murugan, M., Shetty, P.K., Ravi, R., Anandhi, A. and Rajkumar, A.J. 2012. Climate change and crop yields in the Indian Cardamom Hills, 1978- 2007 CE. Climatic Change 110(3–4): 737–753.
Muscolo, A., Sidari, M., Anastasi, U., Santonoceto, C. and Maggio, A. 2014. Effect of PEG-induced drought stress on seed germination of four lentil genotypes. Journal of Plant Interactions 9(1): 354–363.
Nadiya, F., Anjali, N., Gangaprasad, A. and Sabu, K.K. 2015. High-quality RNA extraction from small cardamom tissues rich in polysaccharides and polyphenols. Analytical Biochemistry 485: 25–27.
Pavli, O.I., Ghikas, D.V., Katsiotis, A. and Skaracis, G.N. 2011. Differential expression of heat shock protein genes in sorghum (Sorghum bicolor L.) genotypes under heat stress. Australian Journal of Crop Science 5(5): 511–515.
Pfaffl, M. W., Tichopad, A., Prgomet, C. and Neuvians, T.P. 2004. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: Best Keeper - Excel-based tool using pair-wise correlations. Biotechnology Letters 26(6): 509–515.
Phukan, U.J., Jeena, G.S. and Shukla, R.K. 2016. WRKY transcription factors: Molecular regulation and stress responses in plants. Frontiers in Plant Science 7: 1–14.
Sachs, M.M. and Ho, T.H.D. 1986. Alteration of gene expression during environmental stress in plants. Annual Review of Plant Physiology 37(1): 363 – 376.
Shekhawat, U.K.S., Ganapathi, T.R. and Srinivas, L. 2011. Cloning and characterization of a novel stress-responsive WRKY transcription factor gene (MusaWRKY71) from
Musa spp. cv. Karibale Monthan (ABB group) using transformed banana cells. Molecular Biology Reports 38(6): 4023–4035.
UlHaq, S., Khan, A., Ali, M., Khattak, A.M., Gai, W.X., Zhang, H.X., Wei, A.M. and Gong, Z.H. 2019. Heat shock proteins: Dynamic biomolecules to counter plant biotic and abiotic stresses. International Journal of Molecular Sciences 20(21): 1–31.
Vijayan, A.K. 2018. Small cardamom production technology and future prospects. International Journal of Agriculture Sciences 10(16): 6943–6948.
Wang, A., Yu, X., Mao, Y., Liu, Y., Liu, G., Liu, Y. and Niu, X. 2015. Overexpression of a small heat‐shock‐protein gene enhances tolerance to abiotic stresses in rice. Plant Breeding 134(4): 384 – 393
Wu, M.T. and Wallner, S.J. 1984. Heat Stress Responses in Cultured Plant Cells. Plant Physiology 75(3): 778–780.
Xiong, L., Schumaker, K.S. and Zhu, J.K. 2002. Cell signaling during cold, drought, and salt stress. Plant Cell 14: 165–183.
Ye, J., Coulouris, G., Zaretskaya, I., Cutcutache, I., Rozen, S. and Madden, T.L. 2012. Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics 13(134).
Yu, Y., Zhang, G., Chen, Y., Bai, Q., Gao, C., Zeng, L., Li, Z., Cheng, Y., Chen, J., Sun, X., Guo, L., Xu, J. and Yan, Z. 2019. Selection of reference genes for qPCR analyses of gene expression in ramie leaves and roots across eleven abiotic/biotic treatments. Scientific Reports 9(1): 1–13.
Zagorskaya, A.A. and Deineko, E.V. 2017. Suspension-cultured plant cells as a platform for obtaining recombinant proteins. Russian Journal of Plant Physiology 64(6): 795–807.
Zhu, J.K. 2002. Salt and drought stress signal transduction in plants. Annual Review of Plant Biology 53: 247–273.
Published
How to Cite
Issue
Section
Copyright (c) 2023 Journal of Plantation Crops
This work is licensed under a Creative Commons Attribution 4.0 International License.
.