Micropropagation of Crotalaria laburnifolia L. – An ethnomedicinally important herbal species

Authors

  • K Rajender Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.
  • M Thirupathi Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.
  • D Srinivas Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.
  • D Raju Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.
  • K Jaganmohan Reddy Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

Keywords:

Micropropagation, Crotalaria, Ethanomedicine, Herbal medicine.

Abstract

A protocol for in vitro shoot multiplication in Crotalaria labumifolia L. through nodal explants was established. Excision and culture of the nodal segments from in vitro developed shoots on fresh MS medium with concentration of BA (1mg/1) facilitated development of multiple shoots. Subsequent cultures enhanced the rate of shoot proliferation. Shoots cultured on ½ MS medium containing NAA (0.5 mg/1) initiated roots well compared with IBA and this is the most suitable protocol studied in C. labumifolia. The present study is the first report on in vitro regeneration in this species 

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Author Biographies

K Rajender, Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

M Thirupathi, Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

D Srinivas, Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

D Raju, Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

K Jaganmohan Reddy, Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal – 506 009, India.

Published

30-11-2012

How to Cite

Rajender, K., Thirupathi, M., Srinivas, D., Raju, D., & Jaganmohan Reddy, K. (2012). Micropropagation of Crotalaria laburnifolia L. – An ethnomedicinally important herbal species. Journal of Phytology, 4(4). Retrieved from https://updatepublishing.com/journal/index.php/jp/article/view/2782

Issue

Volume 4, Issue 4, 2012

Section

Research Article