Stress causing dynamic changes of four phytohormones in tobacco and tomato: A GC-MS analysis
Many analytical procedures have been developed to determine the importance of phytohormones in different plants. The work reported here provides a sensitive, accurate and readily accessible gas chromatography coupled to mass spectrometry (GC-MS) technique designed for the simultaneous quantitation of phytohormones indole-3-aceticacid (IAA), abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA). These signalling molecules were analysed in two different plants, Tomato and Tobacco grown in vitro. The protocol designed to assess the dynamic changes in endogenous concentrations of hormones to study plant responses to abiotic stresses in leaf tissues. A hormone profiling is obtained from leaves of plants exposed to salt stress show that different plant hormones are involved in diverse physiologicsl processes. Crosstalk between these hormones result in synergetic or antagonic interactions which have important roles to play in abiotic stress response.