Stress causing dynamic changes of four phytohormones in tobacco and tomato: A GC-MS analysis

  • Chaitali Roy Division of Plant Biology, Bose Institute, 93/1 Acharya Prafulla Chandra Road, Kolkata 700009, India
  • Smriti Ranjan Maji CIF, P-1/12, C.I.T. Scheme VII M, Bose Institute, Kolkata 700054, India
  • Tapas Kumar Ghose, Swati Gupta Bhattacharya Division of Plant Biology, Bose Institute, 93/1 Acharya Prafulla Chandra Road, Kolkata 700009, India

Abstract

Many analytical procedures have been developed to determine the importance of phytohormones in different plants. The work reported here provides a sensitive, accurate and readily accessible gas chromatography coupled to mass spectrometry (GC-MS) technique designed for the simultaneous quantitation of phytohormones indole-3-aceticacid (IAA), abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA). These signalling molecules were analysed in two different plants, Tomato and Tobacco grown in vitro. The protocol designed to assess the dynamic changes in endogenous concentrations of hormones to study plant responses to abiotic stresses in leaf tissues. A hormone profiling is obtained from leaves of plants exposed to salt stress show that different plant hormones are involved in diverse physiologicsl processes. Crosstalk between these hormones result in synergetic or antagonic interactions which have important roles to play in abiotic stress response.

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How to Cite
Roy, C., S. R. Maji, and T. K. G. Swati Gupta Bhattacharya. “Stress Causing Dynamic Changes of Four Phytohormones in Tobacco and Tomato: A GC-MS Analysis”. Research in Plant Biology, Vol. 9, no. 1,  , pp. 16-22, doi:https://doi.org/10.25081/ripb.2019.v9.3753.
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