Studies on isolation, rheological properties and diversity analysis of guar gum

Abstract

studied. Further cetyl trimethyl ammonium bromide (CTAB) method of DNAisolation procedure was standardised to yield good quality of DNA from cluster bean. APCR optimisation with appropriate concentration as well as temperature and timeadjustment was done. Decamer primer used to analyse hybridization study with DNA.The products were run on agarose gel and photographed. The random amplifiedpolymorphic DNA (RAPD) marker was used to study the fifteen genotypes of clusterbean. A total 20 primers were screened out of which 10 have good results. Theamplification of genomic DNA of 15 genotype, using RAPD primers yielded 494fragments that could be scored, of which 55 were polymorphic in all crosses. Per cent ofpolymorphism ranged from 20.51-100%. Similarity matrix obtained through NTSYS-pcsoftware revealed that wild variety NSG-369 shows maximum diversity. The rheologicalproperties of guar gum found to contain galactomannan for its gummy characteristics.Finally it can be concluded that there is no correlation between the diversity of the varietyand its galactomannan content but wild one variety shows great diversity than the othervarieties.