Optimization of process parameters of cyclodextrin glycosyltransferase isolated from novel mutated Bacillus sp. TPR71HNA6 by Taguchi orthogonal array method

Abstract

Cyclodextrin glycosyl transferase CGTase) production by Bacillus SP. TPR71HNA6 under shake flask culture was optimized following Taguchi orthogonal array (OA) design of experiment.  An OA layout of L16 was constructed with five most influensive factors on CGTase like pH, initial temperature, agitation speed, time of incubation and inoculum concentration at three levels for the proposed experimental design. According to the experimental plan (L-16) the experiments were conducted and the data revealed significant variation in the enzyme production. Minimum and maximum CGTase production values were observed to be 19 and 89 U/mL under the above fermentation conditions. The observed variation certainly indicates the imperative role of optimization of all fermentation-related factors in achieving the best possible yields. With the help of statistical tool by optimizing the process variables without altering the nutritional compounds and their concentration an overall 39% of enzyme yield was improved.