In Vitro Meristem Culture and Regeneration of Three Potato Varieties of Bangladesh

Abstract

This paper describes a regeneration protocol by in vitro meristem culture to get disease free potato plantlets. Three recently released varieties of potato (Solanum tuberosum L.) namely Esprit, Lady Rosseta and Meridian were used for this work. In all the three varieties, the percentage of survived explants was the maximum in surface sterilization with 0.1% HgCl₂ for a period of 4 minutes when cultured in MS basal medium. After having DAS-Elisa test, the in vitro grown plantlets were being used for massive micropropagation. In order to get multiple shoot buds in shoot apex and nodal segment and their further elongation, MS medium supplemented with 1.0 mg/l BAP+1.0 mg/l GA₃ was proved best in case of Esprit and Meridian whereas in Lady Rosseta MS medium fortified with 1.0 mg/l BAP+0.5 mg/l GA₃ was the best. Various rooting media were used for induction of well-developed root system in elongated shoot buds. MS medium supplemented with 0.5 mg/l IAA was found appropriate for induction of well root system in all the three varieties.