Optimization of protease production by <em>Bacillus licheniformis</em> in Sugarcane bagasse using statistical experimental design

Authors

  • P. Rathakrishnan Department of Chemical Engineering, Annamalai University, Annamalainagar - 608002, Tamil Nadu, India
  • P. Nagarajan Department of Chemical Engineering, Annamalai University, Annamalainagar - 608002, Tamil Nadu, India
  • R. Rajeshkannan Department of Chemical Engineering, Annamalai University, Annamalainagar - 608002, Tamil Nadu, India

Keywords:

protease, sugarcane bagasse, Bacillus licheniformis, Optimization, RSM

Abstract

Sugarcane bagasse, the residue obtained after extracting the sugar juice from sugarcane was tested for the production of protease under solid-state fermentation (SSF) using Bacillus licheniformis. The fermentation variables were selected in accordance with Plackett-Burman design and were further optimized via response surface methodological approach. Four significant variables (K2HPO4, Beef extract, NaNO3 and Glycine) were selected for the optimization studies. The optimum values for the selected variables were; K2HPO4 -0.3464g/gds, Beef extract- 0.1039g/gds, NaNO3- 0.0334g/gds and Glycine- 0.1027g/gds. A second-order model equation was suggested and then validated experimentally.The model adequacy was very satisfactory as the coefficient of determination was 0.95. The maximum protease production was 146.28U/gds

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Published

02-07-2012

How to Cite

Rathakrishnan, P., Nagarajan, P., & Rajeshkannan, R. (2012). Optimization of protease production by <em>Bacillus licheniformis</em> in Sugarcane bagasse using statistical experimental design. Research in Biotechnology, 3(4). Retrieved from https://updatepublishing.com/journal/index.php/rib/article/view/2409

Issue

Vol. 3 No. 4 (2012)

Section

Research Articles