Micropropagation of <em>Dysophylla myosuroides</em> (Roth.) Benth. In. Wall. through leaf culture

Authors

  • N. Savithramma Department of Botany, Sri Venkateswara University, Tirupati – 517 502, A.P., India
  • S. Ankanna Department of Botany, Sri Venkateswara University, Tirupati – 517 502, A.P., India
  • Beena Prabha Department of Botany, Sri Venkateswara University, Tirupati – 517 502, A.P., India
  • A. Sasikala Department of Botany, Sri Venkateswara University, Tirupati – 517 502, A.P., India

Keywords:

Dysophylla myosuroides, growth hormones, callus induction, shoot proliferation, transplantation

Abstract

A method was developed through leaf as explant of Dysophylla myosuroides (Roth.) Benth. for multiple shoot regeneration. The effect of Auxins (IAA, NAA, IBA) and Cytokinins (BA) were studied on shoot regeneration in clulture. On MS half strength medium light green compact calli were formed with 0.1 mg l-1 NAA. The cultures produced 62.57 + 0.04 shoots on half strength medium with 0.5 mg l-1 BA and 0.01 mg l-1 NAA and also maximum mean length (0.52 + 0.02) of shoots were achieved. In vitro produced shoots rooted on half strength MS medium with 1 mg l-1 IBA. The in vitro regenerated plantlets were successfully acclimatized in paper cups containing vermiculate, then transferred to green house. Hardened plants were transplanted in to sand and soil (1 : 1).

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Published

02-03-2012

How to Cite

Savithramma, N., Ankanna, S., Prabha, B., & Sasikala, A. (2012). Micropropagation of <em>Dysophylla myosuroides</em> (Roth.) Benth. In. Wall. through leaf culture. Research in Biotechnology, 3(2). Retrieved from https://updatepublishing.com/journal/index.php/rib/article/view/2398

Issue

Vol. 3 No. 2 (2012)

Section

Research Articles