Analysis of genetic variability in patchouli cultivars (<em>Pogostemon cablin</em> Benth.) by using RAPD Markers

Abstract

The genetic relationships among patchouli cultivars were determined by using Random Amplified Polymorphic DNA (RAPD) technology. Among 45 decamer random primers used for PCR reactions, 10 primers showed reproducible results. Out of 98 amplification products recorded, 16.7 per cent were monomorphic and 83.3 per cent were polymorphic. The highest dissimilarity (7.35) was detected between KSM 4 and 5 and the least 3.61 between KSM 2 and 3. Dendrogram constructed by cluster analysis of RAPD markers using Unweighted Pair Group Method of Arithmetic Averages  (UPGMA) produced two major clusters ‘A’ and ‘B’. Cluster ‘A’ consisted of five cultivars that segregated into two sub clusters ‘A1’ and ‘A2’. Overall, RAPD analysis revealed the existence of considerable genetic variations in patchouli cultivars. This information regarding genetic variability at the molecular level could be used to identify and develop genetically unique germplasm that complements existing cultivars.