<em>In vitro</em> plant regeneration from leaf explants of <em>Withania somnifera</em> (L) Dunal (Ashwaganda) - an important medicinal plant

Authors

  • Owk Aniel Kumar Department of Botany, Andhra University, Visakhapatnam, India – 530 003
  • Geddam Jyothirmayee Department of Botany, Andhra University, Visakhapatnam, India – 530 003
  • Sape Subba Tata Department of Botany, Andhra University, Visakhapatnam, India – 530 003

Keywords:

Adventitious shoot, Ashwaganda, BAP, IAA, IBA, KN

Abstract

Withania somnifera (L) Dunal (Ashwaganda) is a Solanaceous herb having numerous medicinal values widely used in traditional Ayurvedic drug preparations. An efficient protocol for in vitro plant regeneration via direct adventitious shoot proliferation from leaf explants of Ashwaganda is developed. MS medium containing 1.5mg/l BAP and 1.5mg/l IAA was found to be the best medium for maximum in vitro response i.e., 100(% shooting) and 68(shoots/explant). An improved in vitro shoot bud elongation and rooting was achieved on MS medium fortified with 0.15mg/l GA3 and 5mg/l IBA  respectively. Rooted plants were hardened and transplanted in earthen pots and were showed 80-90% survival during transplantation.

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Published

24-10-2011

How to Cite

Kumar, O. A., Jyothirmayee, G., & Tata, S. S. (2011). <em>In vitro</em> plant regeneration from leaf explants of <em>Withania somnifera</em> (L) Dunal (Ashwaganda) - an important medicinal plant. Research in Biotechnology, 2(5). Retrieved from https://updatepublishing.com/journal/index.php/rib/article/view/2369

Issue

Vol. 2 No. 5 (2011)

Section

Research Articles