Detection of <em>Glutathione S-Transferase</em> gene (GST2, <em>GST3</em>) during induction of somatic embryogenesis in grape (<em>Vitis vinifera</em> L.)

Authors

  • Ravindra B. Malabadi Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India Present address: 5Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada
  • Raju K. Chalannavar Department of Biotechnology and Food Technology, Durban University of Technology, P O Box 1334, Durban 4000, South Africa Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India
  • Neelambika T. Meti Department of Agricultural Biotechnology, Bharati Vidyapeeth University, Pune-Satara Road, Katraj, Pune - 411046, Maharashtra, India
  • Gangadhar S. Mulgund Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India
  • K. Nataraja Department of Botany, Karnatak University, Pavate nagar, Dharwad-580003, Karnataka, India
  • S. Vijaya Kumar Department of Biotechnology, Madanapalle Institute of Technology and Science, Madanapalle-517325, Chitoor, Andhra Pradesh, India
  • Venugopala K. Narayanaswamy Department of Biotechnology and Food Technology, Durban University of Technology, P O Box 1334, Durban 4000, South Africa
  • Bharti Odhav Department of Biotechnology and Food Technology, Durban University of Technology, P O Box 1334, Durban 4000, South Africa

Keywords:

harwad, Gene expression, Karnataka, Somatic embryogenesis, Sonaka, Tas-e-Ganesh, Thompson, transcription factor, Vitis vinifera

Abstract

Glutathione S-transferases (GSTs) are an important group of multifunctional enzymes that belong to diverse multigene families. In plants these enzymes are involved in the detoxification of xenobiotic compounds, herbicide detoxification, and primary and secondary metaboplism and play an important role in plant growth and development. There are several reports that show that the expression of GST is linked to the developmental phases of somatic embryogenesis. This study highlights the detection of transcript abundances of glutathione S-transferase genes GST2 and GST3 in the process of somatic embryogenesis of Vitis vinifera, and the lack thereof in non-embryogenic tissue of leaf cultures of grape (control). These results indicate that the expression of GST2 and GST3 could be used as a molecular signal for the identification of embryogenic cultures during the early development of somatic embryos.

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Published

14-02-2013

How to Cite

Malabadi, R. B., Chalannavar, R. K., Meti, N. T., Mulgund, G. S., Nataraja, K., Kumar, S. V., Narayanaswamy, V. K., & Odhav, B. (2013). Detection of <em>Glutathione S-Transferase</em> gene (GST2, <em>GST3</em>) during induction of somatic embryogenesis in grape (<em>Vitis vinifera</em> L.). Research in Biotechnology, 4(1). Retrieved from https://updatepublishing.com/journal/index.php/rib/article/view/2349

Issue

Vol. 4 No. 1 (2013)

Section

Research Articles

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