Application studies of the halotolerant protease from a newly isolated <em>Virgibacillus dokdonensis</em> VIT P14

Abstract

Protease extracted from halotolerant bacterium - was tested for possible industrial applications. This enzyme was able to dissolve blood clot and coagulated egg within 30 min. The enzyme exhibited substantial keratinolytic activity. It was compatible with all the tested commercial detergents like Rin, Surfexcel, Henko, Tide, Ariel and Technobright and was found to be effective in the removal of blood strains from cotton fabric in the presence of these detergents. The enzyme was compatible with the organic solvents like xylene, toluene, hexane and ethanol and the maximum activity was observed in the presence of ethanol. The enzyme was tested for antimicrobial activity against gram positive and gram negative bacteria and it was found that it possesses good inhibition capability against Escherichia coli, Streptococcus eqiuns, Staphylococcus aureus and Salmonella enterica. The present report indicates that this halotolerant  protease has a wide range of properties and the conditions could be optimized to suit any particular industrial application.