Isolation and identification of a new<em> Bacillus</em> strain for amylase production

Authors

  • Yasser Bakri Department of Molecular Biology and Biotechnology, AECS, Damascus, Syria, P.O.Box 6091
  • Hassan Ammouneh Department of Molecular Biology and Biotechnology, AECS, Damascus, Syria, P.O.Box 6091
  • Samir El-Khouri Department of Molecular Biology and Biotechnology, AECS, Damascus, Syria, P.O.Box 6091
  • Muhanad Harba Department of Molecular Biology and Biotechnology, AECS, Damascus, Syria, P.O.Box 6091
  • Philippe Thonart Centre Wallon de Biologie Industrielle, Unité de Bio-industrie, Faculté de Agro-Biotechnologie de Gembloux, Université de Liège, Passage des Déportes 2, B-5030 Gembloux, Belgium

Keywords:

Bacillus sp, solid culture, α-amylase

Abstract

The objective of the study was to isolate amylase producing bacteria from Syrian soil samples. Amylolytic Bacillus sp were isolated on starch agar medium. The Bacillus SY134D was the best amylase producing isolate among two hundred and ten isolates showed the amylolytic activity. The closest phylogenetic neighbours according to 16S gene sequence, the Bacillus SY134D isolate was identified as Bacillus subtilis. The optimum temperature for amylase production was found to be 37°C. Amylase  production occurred at pH 3.0-9.0 with a maximum at pH 5.0. The best enzyme activity was observed at pH 6.0 and temperature 70°C.

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Published

16-12-2012

How to Cite

Bakri, Y., Ammouneh, H., El-Khouri, S., Harba, M., & Thonart, P. (2012). Isolation and identification of a new<em> Bacillus</em> strain for amylase production. Research in Biotechnology, 3(6). Retrieved from https://updatepublishing.com/journal/index.php/rib/article/view/2347

Issue

Vol. 3 No. 6 (2012)

Section

Research Articles

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